Copyright © 2022 Foshan MBRT Nanofiberlabs Technology Co., Ltd All rights reserved.Site Map
In August 2024, Xie, Mingjun's team published a new research paper in BIO-DESIGN AND MANUFACTURING (impact factor: 8.1), "Rapid fabrication of modular 3D paper-based microfluidic chips using projection-based 3D printing", making important progress in the preparation of microfluidic chips.
Based on the manufacturing method of modular three-dimensional (3D) paper-based microfluidic chips using projection 3D printing (PBP) technology, the research team designed and produced a series of two-dimensional paper-based microfluidic modules; and assembled multiple three-dimensional paper-based microfluidic chips using different methods, demonstrating its modular design, which can be easily assembled and easily disassembled and replaced; in addition, the results of channel flow and cell experiments confirmed that the three-dimensional paper-based microfluidic chips they prepared have good feasibility and biocompatibility.
A novel method for manufacturing microfluidic chips:
Using 3D Electrospinning Machine to manufacture 3D paper-based microfluidic chips.
Figure 1. Schematic diagram of the production of modular 3D paper-based microfluidic chips based on projection 3D printing
Modular design scheme:
The microfluidic chip was modularly designed. Through three assembly methods, namely stacking, rotation and sliding, it was verified that it can be applied in different occasions and has the characteristics of being detachable and easy to replace.
Figure 2. Multifunctional module design of three-dimensional paper-based microfluidic chip
Figure 3.Assembly methods and diffusion testing of 3D paper-based microfluidic chips. a Different assembly methods. Diffusion test of 3D paper-based microfluidic chips based on the b stack, c rotate, and d slide assembly methods. e A complex chip made using stack, rotate, and slide assembly methods and its diffusion test
Successful 3D cell culture system:
A special modular chip was assembled to test the cell culture capabilities of their method, and the results showed that cells could remain active and functional on their assembled microfluidic chip.
Figure 6.2D and 3D cell culture on 3D paper-based microfluidic chips.a Scaffold-based 2D cell culture sketch.b Photos of 3D paper-based microfluidic chips with a scaffold-based 2D cell sample.c Live/dead testing of human umbilical vein endothelial cells (HUVECs) on the poly ε-caprolactone (PCL) scaffolds.d Morphology of HUVECs on the PCL scaffolds stained by phalloidin and 4’,6-diamidino-2-phenylindole(DAPI).e Hydrogel-based 3D cell culture sketch.f Photos of 3D paper-based microfluidic chips with a hydrogel-based 3D cell sample.g Live/dead testing of HUVECs in the gelatin methacryloyl (GelMA) hydrogel.h Morphology of HUVECs in the GelMA hydrogel stained by phalloidin and DAPI
Figure 8.Establishment of the multiorgan microfluidic chip. a Schematic diagram of the multiorgan microfluidic chip. Photos of b the 3D organ structure directly printed on the chip and c the chip itself
This study demonstrates that the modular 3D paper-based microfluidic chips prepared have potential application prospects in a variety of biomedical applications, and provides researchers with novel ideas for new preparation methods of microfluidic chips.
Paper link：https://link.springer.com/article/10.1007/s42242-024-00298-y
