Copyright © 2022 Foshan MBRT Nanofiberlabs Technology Co., Ltd All rights reserved.Site Map
Bone injury and bone defects have always been a difficult problem in the field of clinical treatment. Bone tissue engineering is widely regarded as the most promising method for treating diseased or damaged tissue. The basic strategy of bone tissue engineering includes the development of bioactive implants to repair defective bone tissue. Bone tissue engineering is to combine porous scaffolds with tissue cells and related factors to assist cell growth. This is most effectively achieved by mimicking the morphological characteristics and physical signals of the natural bone extracellular matrix (ECM).
The main point of this paper
Importance of Nanofiber Scaffolds:
Nanofiber scaffolds play an important role in controlling cell behavior, especially in adhesion, proliferation and differentiation, due to their physical properties similar to those of natural extracellular matrix.
Electrospinning Technology:
Electrospinning is an economical and convenient method for preparing nanofibers. It is able to prepare micro/nano-diameter fibers from polymer solution droplets under the action of a strong electric field.
Polymer Selection:
A variety of synthetic polymers can be used for electrospinning, including PCL, PLGA and PLLA. In particular, PLLA, as an environmentally friendly material obtained from natural resources, has good biodegradability and biocompatibility and has been approved by the FDA for biomedical applications.
Application of Magnetic Nanoparticles (MNPs):
Magnetic nanoparticles such as Fe3O4 can promote osteoblast activity and bone formation, increase osteogenic differentiation of osteoblasts and stimulate osteoclast apoptosis.
Development of Magnetic Biodegradable Fiber Materials:
The beneficial effects of nanofiber structure and magnetic field on bone regeneration have inspired the development of magnetic biodegradable fiber materials. MNPs are usually embedded in polymers for tissue engineering and drug release.
Preparation and characterization of PLLA/Fe3O4 nanofibers:
In this study, magnetic PLLA/Fe3O4 Nanofiber Membrane was prepared. Polylactic acid and Fe3O4 nanoparticles were combined by electrospinning technology to prepare Nanofiber Membrane with magnetic properties.
The relevant properties of the composite nanofibers were studied by thermal analysis, infrared spectroscopy and X-ray diffraction analysis.
Material selection:
PLLA (poly-L-lactide) is a promising biomass-based polymer material with good biocompatibility and biodegradability.
The incorporation of Fe3O4 nanoparticles (NPs) can improve osteogenic differentiation and proliferation of cells, regardless of the presence of a static magnetic field (SMF).
Composite preparation:
PLLA and Fe3O4 NP composite fibers were prepared by electrospinning, and the effects of spinning conditions on fiber morphology were explored.
Different PLLA/Fe3O4 mass ratios had significant effects on the magnetic properties and thermal stability of the composites.
Material properties analysis:
The morphology, crystal state, thermodynamic properties and magnetic properties of the electrospun samples were comprehensively analyzed using SEM, TGA, DSC, XRD, FTIR and VSM.
Research results:
It was found that the magnetic properties of PLLA/Fe3O4 composite electrospun fibers increased with the increase of NP content, except for thermal stability.
The fibers prepared by PLLA with Mn = 170,000 have good morphology when electrospun at 12 KV.
Application prospects:
The research results will help promote the further development of PLLA/Fe3O4 composites in the biomedical field, especially in bone tissue engineering.
Application of magnetic nanomaterials:
Magnetic nanomaterials such as Fe3O4 have shown wide application potential in electromagnetic shielding, filtration separation, tissue engineering scaffolds, cell differentiation induction, hyperthermia, drug delivery and other fields due to their unique physical and chemical properties.
Many reports have discussed the effects of magnetic fields on bone regeneration and reconstruction. Magnetic scaffolds may be introduced into biodegradable polymers by Fe3O4 NPs. In this paper, magnetic PLLA/Fe3O4 composite electrospun scaffolds were prepared. First, the factors affecting the electrospinning process, such as molecular weight, concentration of electrospinning solution, and type of electrospinning collector, were discussed. We found that when the molecular weight and solution concentration were low, it was difficult to form stable polymer fibers due to weak interchain interaction forces acting on the polymer. We also found that under the same electrospinning conditions, the uniformity of fiber diameter was significantly affected by the orientation process used. However, the diameter of the electrospun fibers was smaller. Subsequently, FTIR, XRD, DSC, and TGA were used to analyze the effect of Fe3O4 addition. The analysis showed that the addition of Fe3O4 had no effect on the chemical structure, but the thermal stability decreased. We also found that the electrospun fibers had good magnetic response properties, and the magnetic properties increased with the increase of Fe3O4 NP content. In this study, scaffold materials with advantages such as fiber uniformity and magnetic properties were prepared. This result has the potential to be widely used in the manufacture of bone tissue engineering scaffolds.
