Electrospinner: Methacrylate-silk fibroin/nanohydroxyapatite hydrogel helps bone immune regulation and regeneration

Application of 3D printing technology in bone tissue engineering

3D printing technology, especially digital light processing (DLP) 3D bioprinting, has been widely used to manufacture biomimetic functional scaffolds for bone tissue engineering. This technology can control the structure of the scaffold at multiple scales, providing a new strategy for using natural biomaterials as bio-inks for traditional 3D printing materials that rely on polymers and ceramics.

Development of silk fibroin-derived hydrogel biomaterials

Researchers have developed a hydrogel biomaterial derived from silk fibroin (SF) by integrating nanohydroxyapatite (nHA) into the hydrogel to form a composite hydrogel that is rapidly cross-linked upon initiation. This composite hydrogel bio-ink not only improves biocompatibility, but also enhances osteogenesis and chondrogenesis.

Combination of DLP 3D bioprinting with silk fibroin-derived hydrogels

The combination of DLP 3D bioprinting technology and silk fibroin-derived hydrogels provides a new strategy for bone regeneration. This combination takes advantage of the advantages of natural biomaterials, and through precise 3D printing technology, scaffolds with structures and functions closer to natural bone tissue can be manufactured.

M2 macrophage polarization and bone regeneration

Transcriptome analysis showed that this composite hydrogel can promote bone regeneration by inducing M2 macrophage polarization. The role of M2 macrophages in bone healing is complex. Switching M0/M1 macrophages to M2 phenotype through precise strategies is more conducive to bone regeneration.

Connection with electrospinning

Combination of 3D printing and electrospinning: Combining electrospinning technology with 3D printing can achieve precise spatial positioning of nanofiber materials and the construction of complex structures, overcoming the limitation that traditional electrospinning can only generate flat or simple-shaped fiber membranes. This combination opens up new directions for the application of nanofiber materials, especially in personalized medicine and tissue engineering.

Application in cartilage repair: Electrospinning technology also shows great potential in cartilage repair. Nanofiber membranes prepared by electrospinning can highly simulate the composition and structure of extracellular matrix fibers, and can also load drugs to achieve controlled release of drugs, which is of great significance for cartilage repair and regeneration.

Verification of in vivo bone regeneration

The rat femoral defect experiment verified the efficacy of SilMA/nHA in bone regeneration, indicating that this composite hydrogel bioink has broad application prospects in bone tissue engineering.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.mtbio.2024.100962