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Pressure ulcers (PUs) are difficult-to-heal wounds caused by reduced oxygen and nutrient supply due to vascular damage. Vascular repair is the key to addressing this challenge, but current clinical measures are limited. The research of Li Changqing's team at the Army Medical University provides a new treatment method, which is to synthesize nanoparticles (NPs) using silk fibroin and load ferroptosis inhibitor MFGE8 to promote vascular repair and wound healing.
Western blotting was used to detect the expression of ferroptosis-related proteins in PU tissues, and it was found that the level of anti-ferroptosis protein GPX4 was higher in mild PU tissues, while the level of pro-ferroptosis protein P53 was lower.
Gene sequencing results showed that ferroptosis-driving genes were significantly enriched in hypoxic VECs, while vesicle secretion-related genes were significantly enriched in normoxic VECs. EVs secreted by normoxic VECs regulate the role of VECs ferroptosis under hypoxic conditions.
In the development of PU, the expression of CD13 and MFGE8 increased, while the expression of MFGE8 decreased, indicating that they play an important role in the development of PU.
Silk fibroin NPs@MFGE8 can mimic VECs-derived EVs and effectively inhibit hypoxia-induced ferroptosis.
Cross-linked NGR NPs coated with MFGE8 were constructed, and HOOC-PEG-COOH was used as a bridge to cross-link NGR peptide and silk fibroin NPs, effectively carrying MFGE8 into hypoxia-treated VECs.
Hydrogel loaded with NGR-NPs@MFGE8 significantly accelerated ulcer healing in promoting ulcer healing, indicating its potential in PU treatment.
The application of electrospinning technology in this study is mainly reflected in the preparation of silk fibroin-based nanofiber hydrogels. These hydrogels can effectively load and slowly release MFGE8 as carriers, thereby inhibiting the ferroptosis of VECs and accelerating wound healing. In addition, the nanofiber scaffolds prepared by electrospinning technology provide a new way to mimic the function of EVs and achieve more effective treatment by targeting specific cells and molecules.
This study successfully prepared a silk fibroin/collagen hydrogel sustained-release carrier loaded with NGR-NPs@MFGE8, which inhibited the ferroptosis of VECs by targeting CD13 in PU tissue, prevented PU formation, and promoted wound healing. This new method provides a new strategy for the effective treatment of skin PU and has important clinical application prospects.
Overall, this study not only provides new ideas and methods for the treatment of PU, but also provides a new perspective for the application of electrospinning technology in the biomedical field. By combining biomaterials and nanotechnology, more innovative treatment strategies can be developed to address challenges in clinical practice.
Electrospinning Nanofibers Article Source:
https://doi.org/10.1186/s12951-023-02185-7
