Electrospinning Device: Aptamer-free upconversion nanoparticle/silk biosensor system for low-cost and highly sensitive detection of antibiotic residues

Background

The detection of antibiotic residues is essential for protecting the environment, ensuring food safety, and promoting human health. However, the development of rapid, simple, low-cost, and sensitive methods for antibiotic detection faces major challenges. In this study, an aptamer-free biosensor based on Förster resonance energy transfer (FRET) and charge transfer effects was proposed for the detection of roxithromycin (RXM).

Research Methods

UCNPs were synthesized by a modified reverse microemulsion method and hydrophilized to form UCNPs. Then, UCNPs were mixed with SF, and SF was treated with ethanol to form a stable β-folded structure on the surface of UCNPs to construct the SF protein corona-coated UCNPs (UCNPS@SF) complex.

Characterization of Biosensor

The structure and properties of UCNPS@SF were characterized in detail by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), circular dichroism (CD) and other techniques.

Detection principle

Based on the FRET principle, UCNPs are used as energy donors and AR-RXM complexes as energy acceptors, and effective energy transfer is achieved through the regulation of the SF protein canopy.

Research results

The constructed UCNPS@SF biosensor has high detection sensitivity for RXM in deionized water, with a detection range of 1.0 nM to 141.6 nM and a detection limit as low as 0.68 nM. The sensor exhibits long-term stability in aqueous solution (up to 60 days) and no attenuation of fluorescence intensity. In addition, the applicability of the biosensor extends to high-sensitivity detection of other types of antibiotics.

Connection with electrospinning

Environmental and food safety: Electrospinning technology also shows great potential in the fields of environmental protection and food safety. For example, electrospinning technology can be used to prepare nanofibers with high adsorption and barrier properties for environmental pollution control and food safety testing. The silk protein-coated UCNPs biosensor uses Förster resonance energy transfer and charge transfer effects to achieve high-sensitivity detection of antibiotic molecules, which is of great significance for environmental monitoring and food safety.

Technological development and challenges: The research progress, model and simulation of electrospinning technology are also hot topics in current research. For UCNPs biosensors coated with silk protein, understanding the physical model and parameter influence in the electrospinning process is crucial to optimizing the preparation process of silk protein-based nanofibers

Conclusion

The aptamer-free UCNP/SF biosensor developed in this study uses FRET and charge transfer effects to achieve efficient recognition of antibiotic molecules. The system not only shows excellent sensitivity and a wide detection range, but also has long-term stability and environmental friendliness, is easy to operate, and is easy to promote and apply. Its accuracy and reliability were verified by comparison with traditional detection methods, providing an effective detection tool for environmental monitoring, food safety and biomedical fields.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.bios.2024.116335