Electrospinning Device: Silk fibroin/chitin sponge silk fibroin membrane biphasic scaffold based on surgical design for the treatment of osteoarthritis cartilage defects

Application of Silk Fibroin and Chitosan Dual-Phase Scaffolds in Osteoarthritis Surgery

Morphological and structural advantages: The dual-phase scaffold consists of a silk fibroin membrane and a silk fibroin/chitosan sponge, with the silk fibroin membrane attached to the sponge part, and has a dense surface and porous structure. This structure provides both good mechanical strength and is conducive to cell adhesion and growth.

Physical property regulation: By adjusting the ratio of silk fibroin and chitosan, the mechanical properties and expansion rate of the scaffold can be precisely regulated. For example, the SF30 scaffold has the largest pore size distribution range, a low Young's modulus, and a moderate swelling rate, which give it good physical stability and adaptability in osteoarthritis surgery.

Biological function promotion: The dual-phase scaffold can promote the proliferation of rat mesenchymal stem cells (RMSCs) and induce their differentiation into chondrocytes. The SF30 scaffold exhibits suitable morphology, physical stability, and biological functions, which can promote the regulation of RMSCs on chondrocytes, thereby showing the prospect of cartilage regeneration in osteoarthritis surgery.

Application of electrospinning equipment in the preparation of biphasic scaffolds

Preparation of nanofiber structure: Electrospinning equipment can prepare silk fibroin and chitosan nanofibers with specific structures and properties. These nanofibers can be used to construct more sophisticated biphasic scaffold structures and further optimize their performance. For example, nanofibers prepared by electrospinning technology can be compounded with biphasic scaffolds to form composite materials with better mechanical properties and biocompatibility.

Regulating the microstructure of the scaffold: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of the biphasic scaffold to be precisely regulated, thereby better meeting the needs of osteoarthritis surgery.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. For example, growth factors and anti-inflammatory factors that promote cartilage regeneration are loaded into silk fibroin and chitosan nanofibers. By controlling the release rate, the continuous effect of bioactive substances at the surgical site of osteoarthritis can be achieved, further accelerating the repair of cartilage tissue.

Summary

In summary, the silk fibroin and chitosan biphasic scaffolds showed good application effects in osteoarthritis surgery, and the electrospinning equipment provided strong technical support for its preparation. Through electrospinning technology, nanofiber scaffolds with specific structures and properties can be prepared, and the performance of biphasic scaffolds can be further optimized to improve their application effects in osteoarthritis surgery.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.matdes.2018.01.006