Electrospinning Device: Silk-inspired in situ hydrogels with anti-tumor immunity enhance photodynamic therapy for melanoma and infected wounds

Application of silk fibroin hydrogel in melanoma treatment and wound healing

Enhanced anti-tumor effect: The silk fibroin hydrogel system has the ability to recruit macrophages, and can activate anti-tumor immune responses in a controllable manner through near-infrared radiation, achieving combined treatment with photodynamic therapy (PDT), thereby significantly inhibiting the recurrence of melanoma. Under near-infrared irradiation, SFMA-Ce6 hydrogel can effectively kill tumor cells and inhibit tumor growth.

Promote wound healing: The hydrogel system has the ability to light-controllably regulate the phenotype of macrophages, and its effective photodynamic antibacterial activity can promote significant tissue ingrowth and hair follicle regeneration, and accelerate wound healing of Staphylococcus aureus infection. PDT accelerates wound closure by enhancing the differentiation, proliferation and migration of epidermal stem cells, promoting epithelial re-formation and angiogenesis.

Good biocompatibility: SFMA and SFMA-ce6 hydrogels showed good biocompatibility in in vitro experiments, and L929 fibroblasts could proliferate normally on them, and the cell viability exceeded 95%, indicating that these silk-based hydrogels can be used in biomedical applications.

Application of electrospinning equipment in the preparation of silk fibroin hydrogel

Preparation of nanofiber structure: Electrospinning equipment can prepare silk fibroin nanofibers with specific structures and properties. These nanofibers can be used to construct finer hydrogel structures and further optimize their performance. For example, silk fibroin nanofibers prepared by electrospinning technology can be compounded with SFMA-Ce6 hydrogel to form a composite material with better mechanical properties and biocompatibility, providing a more ideal scaffold for melanoma treatment and wound healing.

Regulating the microstructure of hydrogel: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of silk fibroin hydrogel to be precisely regulated, thereby better meeting the needs of melanoma treatment and wound healing, and providing a suitable microenvironment for cell adhesion, growth, and tissue repair.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber hydrogels loaded with bioactive molecules. For example, drugs and growth factors with anti-tumor or healing effects are loaded into silk fibroin nanofibers, and the release rate is controlled to achieve the continuous effect of bioactive substances at the melanoma treatment site or wound site, further improving the treatment effect.

Summary

In summary, silk fibroin hydrogels show good application effects in melanoma treatment and wound healing, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, silk fibroin nanofiber hydrogels with specific structures and properties can be prepared, and the performance of silk fibroin hydrogels can be further optimized to improve their application effects in melanoma treatment and wound healing.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1002/adfm.202101320