Electrospinning Equipment: Injectable silk acid hydrogel loaded with cells

Research progress of silk protein-based cell-loaded hydrogels

Researcher Guo Chengchen of West Lake University proposed an injectable cell-loaded hydrogel system based on silk acid. Silk acid is a carboxylated derivative of natural silk fibroin and shows promising applications in biomedicine. This study prepared silk acid (SA) by efficient carboxylation modification on silk protein through a one-step modification reaction, and found that SA can spontaneously form hydrogels at 37°C in HEPES solution (pH 7.4). SA hydrogel has good biocompatibility and processability, supporting the survival and growth of three different cell lines (L929, HUVEC and rBMSCs).

Application of electrospinning equipment in silk protein-based materials

Electrospinning equipment can produce high-performance nanofibers with high specific surface area, high porosity and good biocompatibility, which are widely used in tissue engineering, drug delivery and other fields. Combining electrospinning technology with the research of silk protein-based cell-loaded hydrogels can further improve the performance and application effects of materials:

Preparation of nanofiber structures: Electrospinning can produce uniform continuous fibers with diameters ranging from nanometers to micrometers. These fibers can be used to construct the enhanced structure of cell-loaded hydrogels and improve their mechanical properties and stability.

Regulation of fiber morphology and arrangement: By adjusting the parameters in the electrospinning process (such as spinning solution concentration, viscosity, electric field strength, etc.), the diameter and morphology of the fibers can be precisely controlled to optimize the performance of the material.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules, and by controlling the release rate, the continuous effect of bioactive substances in tissue engineering sites can be achieved.

Summary and Outlook

As a new type of biomaterial, silk protein-based cell-loaded hydrogels provide an ideal growth environment for cells by simulating the natural environment of the extracellular matrix, showing great potential in the field of tissue engineering and regenerative medicine. Electrospinning equipment plays an important role in the preparation of silk protein-based materials. It can prepare nanofibers with excellent structure and performance to promote cell growth and tissue regeneration. Future research can further explore the combination of electrospinning technology with other emerging technologies, such as 3D printing, nanotechnology, etc., to achieve more complex and sophisticated structural design and functional regulation. In addition, electrospinning process parameters and material selection can be optimized to improve the biocompatibility, mechanical properties and drug loading capacity of nanofibers, and promote the clinical transformation and application of silk protein-based cell-loaded hydrogels in the field of tissue engineering.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1039/D3CC04280D