Electrospinning Equipment: Osteogenic bifunctional peptides combined with silk fibrin membranes effectively induce osteogenesis in vitro and in vivo

Research content and results

Design and verification of bifunctional peptides: The research team developed a new chimeric peptide containing a motif for binding to silk fibroin and an osteogenic motif from osteogenic growth factor (BMP-2). This chimeric peptide can bind to silk fibroin membrane (SFm) and significantly enhance its osteogenic ability.

Surface modification and hydrophilicity optimization: SFm modified by bifunctional peptides (HL@SFm) exhibits a more hydrophilic surface (contact angle of 58.7°), which is better than unmodified SFm and other control groups, which is conducive to cell adhesion and growth.

Cell viability and osteogenic induction: HL@SFm showed good cell compatibility in in vitro experiments, promoting cell migration and differentiation. In in vivo experiments, HL@SFm can significantly induce bone tissue formation and show excellent osteogenic ability.

In vivo biocompatibility and immunohistochemical analysis: HL@SFm can promote the expression of bone matrix proteins after implantation without causing obvious inflammatory response, showing good tissue compatibility.

Research significance

This study successfully functionalized the silk fibroin membrane by designing specific bifunctional peptides, enabling it to effectively induce osteogenic differentiation, providing a new biomaterial strategy for bone tissue engineering. This strategy not only improves the osteogenic ability of SFm, but also retains its biocompatibility and biodegradability, providing new possibilities for future bone tissue regeneration and repair.

Connection with electrospinning technology

Material functionalization: The research of Professor Yang Mingying's team demonstrated a method of modifying silk fibroin membranes by bifunctional peptides, which can be combined with electrospinning technology to further optimize the surface properties and functions of silk fibroin fibers.

Multifunctionality of biomaterials: Electrospinning technology can prepare nanofibers with high specific surface area and excellent mechanical properties. Combined with bifunctional peptide-modified silk fibroin, more multifunctional fiber materials with biocompatibility and osteogenic ability can be developed.

Application expansion: This combination strategy can not only be used in bone tissue engineering, but also can be extended to other biomedical fields such as drug delivery and tissue repair.

Summary

In summary, the research of Professor Yang Mingying's team provides new ideas for the functionalization of silk fibroin-based biomaterials, and also provides a new direction for the application of electrospinning technology in the biomedical field.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1021/acsami.2c17554