Electrospinner: Extracellular matrix-modified silk cellulose scaffolds regulate brown adipose stem cell cardiogenesis via TGF-β pathway

Background

This study successfully developed a new hybrid scaffold based on silk cellulose (SF) scaffold and cardiac fibroblasts (CFs) derived extracellular matrix (ECM). This scaffold was prepared by physical and chemical double cross-linking network, showing ideal honeycomb structure, hydrophilicity, elasticity and enhanced mechanical properties, which is of great significance for tissue engineering, especially cardiac tissue regeneration. The following is a summary of the research content and findings

Preparation and characterization of ECM-modified SF scaffold

CFs-derived ECM-coated SF scaffolds were generated by inoculating CFs on SF scaffolds and culturing and decellularizing them. This scaffold contains ECM components with myocardial-like properties, such as collagen, laminin, and fibronectin

The effect of ECM on the differentiation of BASCs into CMs

The study found that CF-derived ECM-coated scaffolds increased the expression of CM-specific proteins (such as cardiac troponin T and α-actin) in BASCs, promoting the differentiation of BASCs into CMs

Involvement of signaling pathways

The study revealed the key role of the β1-integrin-dependent transforming growth factor-β1 (TGF-β1) signaling pathway in CF-derived ECM regulating the differentiation of BASCs into CMs

Application of electrospinning technology in tissue engineering

Electrospinning technology has been widely used in tissue engineering due to its high surface area to volume ratio and ability to simulate extracellular matrix, promoting cell migration, proliferation, adhesion and differentiation

EC Advantages of M-modified SF scaffolds

Compared with pure SF-SEAs, Fr-SF-SEAs have better hydrophilicity, modulus, elasticity and stability, and uniform high-cell density regenerated tissues can be quickly obtained through cyclic compression cell seeding mode

Clinical translation potential

Ear-shaped Fr-SF-SEAs achieve stable and precise ear bionic cartilage regeneration at human scale, showing clinical translation potential in otic reconstruction

Biomimetic manufacturing and theoretical basis

These findings provide insights into the biomimetic manufacturing of engineered cardiac tissues (ECTs) and lay a theoretical foundation for the construction of ECTs

In summary, this study developed a new type of scaffold by combining silk fibroin and cardiac fibroblast-derived ECM, which not only promoted the differentiation of stem cells into cardiomyocytes, but also provided an effective platform strategy for cardiac tissue regeneration. In addition, the application of electrospinning technology further expanded the potential of this scaffold in tissue engineering, especially in cardiac tissue repair and regeneration.

Electrospinning Nanofibers Article Source:

https://academic.oup.com/rb/article/7/4/403/5824950