Electrospinner: Enzymatic degradation of low-temperature self-assembled silk fibroin sponges provides a platform for exosome therapy

Background

This study successfully developed a silk fibroin-based cryosponge for controlled release of exosomes, providing a new platform for drug delivery in tissue engineering. The following is a summary of the research content and findings

Preparation of silk fibroin cryosponge

The study describes a new platform for preparing cryosponges through self-assembly of silk fibroin to achieve long-term stable release of bone marrow mesenchymal stem cell (BMSC)-derived exosomes and scaffold degradation

Analysis of silk fibroin structural transformation

With the extension of annealing time, more SF proteins are converted to α-helical structures with β-coils, and this structural transformation has an important effect on the release characteristics of exosomes

Maintenance of exosome structural integrity

The cryosponge maintained the integrity of the exosome structure during long-term storage, which is of great significance for the clinical application of exosomes in tissue engineering

Verification of in vivo function

In vivo experiments showed that exosomes stayed in the undegraded silk fibroin sponge material for two months, which is better than silk fibroin glue, which is often used as a clinical biomaterial. The sponge group containing exosomes showed better neovascularization and tissue growth effects

Relationship with electrospinning

Promote cell migration and growth:

The porous nature of the electrospun nanofiber platform allows the penetration of cells and nutrients, enhancing the overall healing process. This property is particularly important for exosome release and cell migration, neovascularization, and myofibroblast growth in silk fibroin cryosponge.

Material and structural regulation of electrospinning technology:

Electrospinning technology can effectively regulate the fine structure of fibers, which is crucial for the preparation of silk fibroin cryosponge, because this sponge requires specific mechanical properties and degradation curves to achieve long-term stable release of exosomes

Conclusion

Silk fibroin cryosponge provides a new platform for the study of exosome therapy. The effectiveness of this exosome encapsulation strategy has been demonstrated by achieving sustained release and maintaining exosome bioactivity, providing a new direction for exosome-related long-term tissue rehabilitation and regeneration applications.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.bioactmat.2021.06.017