Electrospinner: Injectable silk-based hydrogels for regeneration of critical-sized bone defects

Hydrogel and bone regeneration

OxSF/ACP/PRP hydrogel was developed to mimic the natural bone healing process and serve as a seedbed for bone regeneration. This hydrogel combines oxidized silk fibroin and fibrin as rigid geogrids, amorphous calcium phosphate (ACP) and platelet-rich plasma (PRP) as fertilizer and soil, respectively, exhibiting excellent mechanical and biomineralization properties and promoting vascularized bone regeneration in vivo.

PI3K-Akt signaling pathway

The hydrogel exerts succinate-like functions through the PI3K-Akt signaling pathway, coordinating mitochondrial calcium uptake, converting exogenous ACP into endogenous ACP, and promoting osteogenesis. This mechanism is crucial for bone repair and regeneration because it involves key regulation of cell proliferation, differentiation, and survival.

Application of electrospinning technology

Electrospinning technology is widely used to prepare nanofibers, which are widely used in medicine, environmental protection and other fields. Especially in tissue engineering, electrospun nanofibers are used to simulate extracellular matrix and promote cell growth and tissue repair due to their unique physicochemical properties.

Potential connection between OxSF/ACP/PRP hydrogel and electrospinning

Considering the role of OxSF/ACP/PRP hydrogel in bone regeneration, combined with electrospinning technology, novel silk-based hydrogel composites can be developed. These composites can serve as novel biomineralized seedbeds for bone defect repair, provide early mechanical support and promote bone tissue regeneration.

Application of electrospinning technology in bone repair

Electrospinning technology can be used to prepare nanofibers with specific structures and functions, which can be used as scaffold materials for bone repair and regeneration. For example, composites of silk fibroin and amorphous calcium phosphate can be prepared by electrospinning technology to improve their biocompatibility and mechanical properties.

Future development

The combination of electrospinning technology and OxSF/ACP/PRP hydrogel provides a new idea for the development of novel bone repair materials. Future research can explore how to optimize the fiber structure of hydrogels by electrospinning technology to improve their performance and effect in bone defect repair.

In summary, the combination of OxSF/ACP/PRP hydrogel and electrospinning technology provides a new strategy for bone defect repair and regeneration, which is expected to achieve better bone repair effects by simulating the natural bone healing process and providing a biomineralization environment.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.bioactmat.2024.01.024