Electrospinner: Preparation of hydroxyapatite/silk fibroin composite scaffolds with porous structure and mechanical strength similar to cancellous bone by electric field induced gelation technology

Multi-level porous structure

The e-HA/SF composite scaffold has a biomimetic multi-level porous structure with a porosity similar to that of cancellous bone, which helps to improve cell adhesion and proliferation, thereby promoting bone tissue regeneration

Bioactivity and cell response

The introduced bioactive nano-HA triggered the best cell response in static and dynamic cell culture experiments in vitro, showing good biocompatibility

Biodegradability and biocompatibility

The results showed that the prepared e-HA/SF composite scaffold has moderate biodegradability and good biocompatibility, which is crucial for bone tissue regeneration

Advantages of electric field induced gel technology

Compared with traditional Compared with freeze-drying strategy, electric field induced gelation technology provides a new way to construct bone scaffold materials with enhanced mechanical properties and bioactivity

Structure and composition relationship

The e-HA/SF composite scaffold developed in this study better balances the contradiction between porous structure and mechanical and degradation properties, providing an important scientific basis for studying the structure and composition relationship of HA/SF composite scaffolds

Relationship with electrospinning

LLPS mechanism of silk protein and electrospinning:

Studies have shown that calcium ions (Ca2+) induce LLPS of SF by increasing the hydrophobic and electrostatic interactions between SF under macromolecular crowding conditions. This discovery reveals the key mechanism of silk protein in the natural spinning process and provides a theoretical basis for simulating the natural silk spinning process. Electrospinning technology can use this mechanism to precisely control the phase separation process of SF solution by adjusting the electric field strength and solution properties, thereby preparing nanofibers with specific structures and properties.

Application of electrospinning technology:

Electrospinning technology has been widely used in biomedical materials, filtration and protection, catalysis, energy, optoelectronics, food engineering and other fields. Especially in the biomedical field, nanofibers prepared by electrospinning technology are used in tissue engineering, drug controlled release, wound repair, etc. due to their high specific surface area and porous structure. These characteristics make electrospinning technology have great application potential in bone tissue engineering, especially in the preparation of bone scaffold materials with good cell compatibility and mechanical properties.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1021/acsami.4c12470