Electrospinning Device: 3D printed platelet-rich plasma treated silk composite scaffolds for bone tissue engineering

Application of 3D printed hybrid scaffolds based on silk fibroin combined with PRP post-treatment in bone tissue engineering Promote cell growth and proliferation

PRP treatment can significantly promote the cell growth and proliferation of human adipose-derived mesenchymal stem cells (HADMSCs). One of the potential reasons is that PRP contains a variety of growth factors, such as VEGF, bFGF, PDGF-AA, TGFB3, etc. These growth factors contribute to the growth and proliferation of osteoblasts and mesenchymal stem cells during bone tissue regeneration.

Enhance osteogenic differentiation

PRP treatment significantly upregulated the gene expression levels of late osteogenic markers, such as OCN and OPN, but had no significant effect on the expression of early osteogenic markers (i.e., ALP and Runx2). This indicates that the PRP-treated composite scaffold promotes the proliferation of HAMSCs and the expression of late osteogenic markers, thereby enhancing osteogenic differentiation.

Improve scaffold performance

More small pores appeared on the surface of the composite scaffold after PRP treatment, and the surface morphology was relatively rough. This change in surface structure is conducive to cell adhesion and growth, providing a better microenvironment for bone tissue regeneration.

Application of electrospinning equipment in the preparation of 3D printed hybrid scaffolds based on silk fibroin

Preparation of nanofiber scaffolds: Electrospinning equipment can prepare silk fibroin nanofibers with specific structures and properties. These fibers can be used to construct more sophisticated scaffold structures and further optimize the performance of 3D printed hybrid scaffolds based on silk fibroin. For example, silk fibroin nanofibers prepared by electrospinning technology can be composited with 3D printed SF/GEL/HA/TCP hydrogel scaffolds to form composite materials with better mechanical properties and biocompatibility.

Regulating the microstructure of the scaffold: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of 3D printed hybrid scaffolds based on silk fibroin to be precisely regulated, thereby better meeting the needs of bone tissue regeneration.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. By adjusting the spinning parameters, gradient synthesis can be achieved to obtain fiber membranes of different diameters and different arrangement distributions. This enables the 3D printed hybrid scaffold based on silk fibroin to more effectively deliver bioactive substances such as growth factors and promote the proliferation and osteogenic differentiation of HADMSC.

Summary

In summary, the 3D printed hybrid scaffold based on silk fibroin combined with PRP post-treatment shows good application effect in bone tissue engineering, and the electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber scaffolds with specific structures and properties can be prepared, and the performance of 3D printed hybrid scaffolds based on silk fibroin can be further optimized to improve its application effect in bone tissue engineering.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.bioactmat.2019.09.001