Electrospinning Device: Cell-derived extracellular matrix-modified silk-cellulose scaffolds for brown adipose stem cell cardiogenesis by modulating the TGF-β pathway

Mechanism of action of cell-derived ECM-modified SF scaffolds in cardiomyocyte differentiation

Provide a natural cell growth microenvironment: CF-derived ECM contains ECM components such as collagen types I, III, and IV, fibronectin, and laminin, which can provide BASCs with a natural extracellular matrix environment similar to myocardial tissue and promote cell adhesion and growth.

Promote BASCs to differentiate into cardiomyocytes: CF-derived ECM-coated scaffolds increased the expression of CM-specific proteins (such as cardiac troponin T and α-actin) of BASCs, indicating that it can effectively induce BASCs to differentiate into cardiomyocytes.

Activate TGF-β1 signaling pathway: The β1-integrin-dependent transforming growth factor-β1 signaling pathway participates in the regulation of CF-derived ECM by promoting the differentiation of BASCs into cardiomyocytes.

Application of electrospinning equipment in the preparation of ECM-modified SF scaffolds

Preparation of nanofiber scaffolds: Electrospinning equipment can prepare silk protein nanofibers with specific structures and properties. These fibers can be used to construct more sophisticated scaffold structures and further optimize the performance of ECM-modified SF scaffolds. For example, the silk protein nanofibers prepared by electrospinning technology can be combined with ECM-modified SF scaffolds to form a composite material with better mechanical properties and biocompatibility, providing a more ideal scaffold for myocardial tissue engineering.

Regulating the microstructure of the scaffold: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting the concentration, viscosity, electric field strength and other parameters of the spinning solution. This allows the pore structure and pore size of the ECM-modified SF scaffold to be precisely regulated, thereby better meeting the needs of myocardial cell growth and differentiation.

Loading bioactive substances

Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. By adjusting the spinning parameters, gradient synthesis can be achieved to obtain fiber membranes of different diameters and different arrangement distributions. This enables the ECM-modified SF scaffold to more effectively deliver bioactive substances such as growth factors, promote the proliferation of BASCs and differentiation into myocardial cells.

Summary

In summary, cell-derived ECM-modified SF scaffolds play an important role in myocardial cell differentiation, and electrospinning equipment provides strong technical support for their preparation. Through electrospinning technology, nanofiber scaffolds with specific structures and properties can be prepared, further optimizing the performance of ECM-modified SF scaffolds and improving their application in myocardial tissue engineering.

Electrospinning Nanofibers Article Source:

https://academic.oup.com/rb/article/7/4/403/5824950