Electrospinning Device: Analysis of the molecular mechanism of silk peptide-induced apoptosis in HepG2 cells

Preparation of silk fibroin nanofibers by electrospinning

Electrospinning technology is widely used to prepare silk fibroin nanofibers, which show great potential in the biomedical field, especially in tissue engineering and drug delivery systems due to their unique physical and chemical properties. For example, SF/PLGA nanofiber scaffolds prepared by electrospinning technology show good cell compatibility in vascular tissue engineering, and HUVECs cells can adhere and proliferate well on their surface.

Effect of silk fibroin nanofibers on cell proliferation

Studies have shown that the rate of protein conformational transition in silk fibroin hydrogels has an effect on the proliferation of cells adhering to their surfaces. This means that the surface properties and structure of silk fibroin nanofibers prepared by electrospinning technology may have a regulatory effect on cell proliferation.

Toxicity test of silk fibroin nanofibers

In the application of silk fibroin nanofibers prepared by electrospinning, the study of their toxicity is crucial. Cytotoxicity was evaluated by BrdU analysis, XTT analysis, LDH analysis and live-dead staining, and it was found that silk fibroin nonwovens prepared under specific conditions showed no signs of cytotoxicity.

Multifunctionality of Silk Fibroin Nanofibers

Electrospinning technology can prepare silk fibroin nanofibers with different functions, such as electroactivity, piezoelectricity, etc. These functional nanofibers have potential applications in energy harvesting, sensors and other fields, and also provide a new way to study the cell proliferation effects and toxicity of silk fibroin and its hydrolysates.

Biocompatibility of Silk Fibroin Nanofibers

Silk fibroin nanofibers prepared by electrospinning have good biocompatibility. For example, chitosan-polyethylene oxide-silk fibroin (CS-PEO-SF) nanofibers cross-linked by EDC-NHS showed good cell compatibility and no cytotoxicity.

Cell Proliferation and Apoptosis Effects of Silk Fibroin Hydrolysate (SFP)

The effects of silk fibroin hydrolysate (SFP) on cell proliferation and apoptosis have been explored in studies. SFP dose-dependently promoted the apoptosis of RAW264.7 osteoblasts and MCF-7 breast cancer cells, while in the A549 cancer cell line, SFP induced S phase cell cycle arrest and then promoted cell apoptosis. These findings suggest that the bioactivity of SFP may have an impact on the application of silk fibroin nanofibers prepared by electrospinning.

Summary

In summary, electrospinning technology plays an important role in the study of toxicity and cell proliferation effects of silk fibroin and its hydrolysates. Silk fibroin nanofibers prepared by electrospinning technology not only show positive results in biocompatibility and cell proliferation, but also their multifunctionality provides new possibilities for biomedical applications. At the same time, the study of the cell proliferation and apoptosis effects of SFP also provides an important biological basis for the application of electrospun silk fibroin nanofibers.