Electrospinning Device: Biomimetic AgNPs@antimicrobial peptide/silk fibroin coating for infection-triggered antimicrobial and enhanced osseointegration

Background

The research team led by Yufeng Zheng of Peking University designed an antimicrobial peptide (AMP) with osteogenic fragments, and complexed it on the surface of silver nanoparticles (AgNP) through hydrogen bonds, and used biomimetic silk fibroin (SF) to carry AgNPs@AMPs to achieve infection-triggered antibacterial and bone integration.

Research design and innovation

Yufeng Zheng's team designed an antimicrobial peptide (AMP) with osteogenic fragments, formed a protein crown structure on the surface of silver nanoparticles (AgNPs), and constructed an Ag@AP/SF coating. This design utilizes the synergistic effect of AMPs and AgNPs, and obtains Ag nanoparticles by in-situ reduction of silver ions by SF and carries AgNPs@AMPs, achieving infection-triggered antibacterial and bone integration.

Antibacterial and bone integration ability

The SF-based coating loaded with AgNPs@AMPs exhibits the ability to synergistically inhibit bacteria and promote bone integration. Experiments have verified that AMPs contribute to the dispersion and size regulation of AgNPs, the anion release of the SF-based coating has pH-responsive characteristics, and the coating has excellent long-lasting bactericidal effect.

In vitro and in vivo experimental verification

By co-culturing with BMSCs in vitro and implanting into rat femurs, its in vitro osteogenic ability and in vivo bone integration ability were verified. The results showed that the Ag@AP/SF coating enhanced the cell adhesion, proliferation and osteogenic ability of BMSCs, and in vivo implantation studies confirmed that the Ag@AP/SF coating could promote bone formation and healing.

Connection with electrospinning

Preparation and application of nanofibers: Electrospinning technology can directly and continuously prepare polymer nanofibers, which is essential for the preparation of nanofiber materials with specific functions. In the research of Zheng Yufeng's team, nanofibers for the surface of orthopedic implants can be prepared by electrospinning technology. These fibers can carry AgNPs@AMPs to achieve antibacterial and bone integration functions.

Synergistic effect of antibacterial and osteogenic ability: Nanofibers prepared by electrospinning technology have high specific surface area and porous structure, which make them have potential applications in antibacterial and osteogenic aspects. In the research of Zheng Yufeng's team, the synergistic effect of AgNPs@AMPs provided excellent antibacterial effect and promoted bone integration, which echoed the application of electrospinning technology in the biomedical field.

Application prospects

As a surface modification coating for orthopedic implants, the Ag@AP/SF coating in this study has high bactericidal and osteogenesis ability, showing great application prospects in the treatment of bone defects and related infections.

Research significance

This study provides a new method to solve the infection problem associated with orthopedic implants and promote bone integration, which is of great significance for improving the success rate of implants and reducing postoperative complications. In summary, the research of Zheng Yufeng's team provides an effective solution for the surface modification of orthopedic implants through the innovative AgNPs@AMPs-SF system, which has important clinical application value.

Electrospinning Nanofibers Article Source:

http://doi.org/10.1016/j.bioactmat.2022.05.015