Electrospinning Device: Enzymatic degradation of low-temperature self-assembled silk fibroin sponges provides a platform for exosome therapy

Application of Silk Fibroin Cryosponge in Tissue Engineering

Long-term stable release of exosomes: Silk fibroin cryosponge can achieve long-term stable release of exosomes. Annealing above the glass transition temperature, silk fibroin chains self-assemble into silk fibroin type I structure under freezing conditions. The release of exosomes is enzyme-responsive, and its rate is mainly determined by the enzymatic degradation of the scaffold. In vivo experiments showed that exosomes stayed in the undegraded silk fibroin sponge material for two months.

Promoting tissue repair and regeneration: The silk fibroin cryosponge group containing exosomes showed better neovascularization and tissue growth effects after subcutaneous implantation in nude mice. This shows that silk fibroin cryosponge can effectively promote cell migration and new blood vessel formation, as well as the growth of myofibroblasts.

Maintaining the structural integrity of exosomes: Silk fibroin cryosponge can maintain the structural integrity of exosomes during long-term storage. When stored at -80°C, the exosomes released from the sponge retain their original shape.

Application of electrospinning equipment in the preparation of silk protein cryosponge

Preparation of nanofiber structure: Electrospinning equipment can prepare silk protein nanofibers with specific structure and properties. These nanofibers can be used to construct finer cryosponge structures and further optimize their performance. For example, silk protein nanofibers prepared by electrospinning technology can be compounded with cryosponge to form composite materials with better mechanical properties and biocompatibility.

Regulating the microstructure of sponge: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of silk protein cryosponge to be precisely regulated, thereby better meeting the needs of tissue engineering.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber sponges loaded with bioactive molecules. For example, growth factors and anti-inflammatory factors that promote tissue repair are loaded into silk protein nanofibers, and the release rate is controlled to achieve the continuous effect of bioactive substances in tissue engineering sites, further accelerating tissue repair and regeneration.

Summary

In summary, silk fibroin cryosponge has shown good application effects in tissue engineering, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber sponges with specific structures and properties can be prepared, and the performance of silk fibroin cryosponge can be further optimized to improve its application effect in tissue engineering.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.bioactmat.2021.06.017