Electrospinning Device: In situ injection of silk fibroin hydrogel promotes skin regeneration in full-thickness burn wounds

Application of silk fibroin hydrogel in burn wound treatment

Promoting cell proliferation and migration: Silk fibroin hydrogel showed superiority in in vitro experiments, supporting the proliferation of primary human dermal fibroblasts and the migration of keratinocytes. This is crucial for wound healing, because cell proliferation and migration are the basic processes of wound repair, which can accelerate wound closure and tissue regeneration.

Guiding tissue regeneration: In in vivo experiments, silk fibroin hydrogel provided guidance and support matrix for full-thickness repair of third-degree burn wounds. By promoting the deposition and remodeling of type I and type III collagen fibers, silk fibroin hydrogel can guide the regeneration of overall tissue and restore the structure and function of the skin.

Regulating inflammatory response: By observing the expression of TNF-α and CD163 genes, it can be seen that silk fibroin hydrogel can promote the transition of wounds from the inflammatory stage to the proliferation stage. This helps to reduce the damage to tissues caused by inflammatory reactions and create a good microenvironment for tissue repair and regeneration.

Application of electrospinning equipment in the preparation of silk fibroin hydrogel

Preparing nanofiber structure: Electrospinning equipment can prepare silk fibroin nanofibers with specific structures and properties. These nanofibers can be used to construct finer hydrogel structures and further optimize their performance. For example, silk fibroin nanofibers prepared by electrospinning technology can be compounded with hydrogels to form composite materials with better mechanical properties and biocompatibility, providing more stable support for burn wounds.

Regulating the microstructure of hydrogels: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of silk fibroin hydrogels to be precisely regulated, thereby better meeting the needs of burn wound treatment and providing a suitable microenvironment for cell adhesion, growth, and tissue regeneration.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber hydrogels loaded with bioactive molecules. For example, growth factors and drugs that promote tissue repair are loaded into silk fibroin nanofibers and then combined with hydrogels. By controlling the release rate, the continuous effect of bioactive substances on burn wounds can be achieved. This can not only enhance the biological function of hydrogels, but also further improve their effect in burn wound treatment.

Summary

In summary, silk fibroin hydrogel has shown good application effects in the treatment of burn wounds, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, silk fibroin nanofiber hydrogel with specific structure and properties can be prepared, and the performance of silk fibroin hydrogel can be further optimized to improve its application effect in the treatment of burn wounds.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1002/adhm.201801092