Electrospinning Device: Self-supporting Nanofiber Membrane Assembled from the Interface Induced by Strong Interaction between Silk Fibroin and Lysozyme Molecules

Study on the Co-assembly of Nanofiber Membrane by Silk Fibroin and Lysozyme Interface

Formation of Stable Co-assembly Protein Nanofiber Membrane: At room temperature, lysozyme (Ly) and TCEP solution were introduced into silk fibroin (SF) aqueous solution. After a period of incubation, a free-floating SF/Ly Nanofiber Membrane was spontaneously formed at the air/solution interface. The membrane will not degrade or dissolve again even if it remains stably suspended for a long time.

Excellent optical and mechanical properties: SF/Ly Nanofiber Membrane has excellent optical transparency, with a transmittance of more than 92% in the entire visible light spectrum. Its Young's modulus is about 6-7GPa, which is 10 times that of nanomembranes without SF.

Versatility: SF/Ly Nanofiber Membrane can be easily transferred from the air/water interface to the air to form a complete independent nanomembrane. Its rich surface functional groups allow for superior adhesion to the underlying substrate, adapting to complex 3D topological surfaces, elastic substrates and even porous skeletons.

Application of electrospinning equipment in the preparation of silk protein Nanofiber Membrane

Preparation of nanofiber structure: Electrospinning equipment can prepare silk protein nanofibers with specific structure and properties. These nanofibers can be used to construct more sophisticated nanomembrane structures and further optimize their performance. For example, silk protein nanofibers prepared by electrospinning technology can be compounded with lysozyme to form a composite Nanofiber Membrane with better mechanical and optical properties.

Regulating the microstructure of nanomembrane: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of the silk protein nanomembrane to be precisely regulated to better meet the needs of different applications.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber membranes loaded with bioactive molecules. For example, bioactive molecules with specific functions are loaded into silk protein nanofibers, and then combined with lysozyme. By controlling the release rate, the continuous action of bioactive substances in the Nanofiber Membrane is achieved.

Summary

In summary, the study of the synergistic assembly of nanofilms at the interface of silk fibroin and lysozyme provides a new idea for the preparation of high-performance nanofilms, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, silk fibroin nanofiber membranes with specific structures and properties can be prepared, and the performance of silk fibroin nanofilms can be further optimized to expand their applications in flexible photonics, electronics, smart skin devices and other fields.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1002/adfm.202312324