Electrospinning Device: Multi-omics analysis of silk fibroin and sericin differentially regulating stem cell paracrine and regenerative functions

Background

Silk, especially silk fibroin (SF) and sericin (SS), has shown great potential in the field of tissue engineering and regenerative medicine due to its high biocompatibility, excellent mechanical properties and controllable degradability. Silk materials are composed of amino acids, which can provide biochemical signals to affect cell behavior and function, thereby regulating tissue repair and regeneration.

Interaction between silk materials and stem cells

Through multi-omics technology, researchers comprehensively analyzed the effects of SF and SS on the biological processes and signaling pathways of human mesenchymal stem cells (MSCs). The study found that SF and SS significantly enhanced the paracrine function of MSCs through the Integrin/PI3K/Akt and glycolysis signaling pathways, involving key tissue regeneration processes such as extracellular matrix deposition, angiogenesis and immune regulation.

Application of electrospinning technology in silk materials

Electrospinning technology can prepare silk materials into electrospun nanofiber scaffolds with different structures, and is widely used in the fields of tissue engineering and regenerative medicine. Electrospinning oriented nanofiber scaffolds have highly consistent fiber arrangement directions, which can promote cell adhesion and migration through contact guidance, further promote cell proliferation and differentiation, and achieve tissue regeneration.

Clinical application prospects of silk materials

Silk functional scaffolds have been designed for the regeneration and repair of various tissues, showing excellent clinical transformation potential and broad clinical application prospects. The study of intrinsic biochemical signals of silk materials will help further clinical transformation.

Multi-omics analysis of silk materials

The development of multi-omics technology makes it possible to comprehensively analyze the tissue cell response mediated by the intrinsic biochemical signals of silk materials and their deep mechanisms. The application of this technology provides a more comprehensive insight into the application of silk materials in tissue engineering and stem cell therapy.

Antibacterial and anti-inflammatory properties of silk materials

Silk materials also show antibacterial and anti-inflammatory properties, which are particularly important for surgical sutures. The antibacterial and anti-inflammatory suture samples mentioned in the study accelerated the disappearance of inflammation in an inflammatory environment, had anti-inflammatory ability, and demonstrated good biocompatibility and surgical suturing properties.

Summary

In summary, silk materials have broad application prospects in the fields of tissue engineering and regenerative medicine, especially when combined with electrospinning technology, nanofiber scaffolds with specific structures and functions can be prepared, providing new solutions for tissue repair and regeneration. Meanwhile, multi-omics analysis of silk materials provides in-depth insights into the molecular mechanisms of their interactions with cells, which helps to optimize the design and application of silk-based scaffolds.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1002/adma.202210517