Electrospinning Device: Nanosilicate-reinforced silk hydrogel for endogenous regeneration of osteochondrocytes

Background

Electrospinning technology is widely used in regenerative medicine because of its advantages such as being able to simulate the natural structure of the extracellular matrix. This technology can produce nanofibers with high porosity and high specific surface area, which are conducive to cell attachment, proliferation and differentiation, and are particularly important for the repair of osteochondral defects.

Advantages of electrospinning oriented nanofibers

Electrospinning oriented nanofiber scaffolds have highly consistent fiber arrangement directions, which can promote cell adhesion and migration through contact guidance, and the combination with cells or growth factors can further promote cell proliferation and differentiation, and ultimately achieve tissue regeneration. It has shown great application potential and broad application prospects in the fields of nerve, myocardium, tendon, bone tissue regeneration and wound healing.

Silk fibroin-montmorillonite composite nanoactive hydrogel

This study prepared a silk fibroin-montmorillonite composite nanoactive hydrogel with dual bone and cartilage activity for integrated osteochondral regeneration. Silk fibroin has good biocompatibility, excellent mechanical properties and adjustable degradation properties, and is widely used in the field of tissue engineering. The introduction of montmorillonite enhances the mechanical properties and osteogenic activity of the hydrogel, and significantly improves the porosity, mechanical properties and hydrophilicity of the material.

Proteomic analysis

Proteomic analysis shows that multiple signaling pathways are involved in regulating the bone/cartilage dual-lineage activity of the hydrogel. It can induce osteogenic differentiation of bone marrow mesenchymal stem cells and maintain the chondrocyte phenotype in vitro, and promote the repair and regeneration of cartilage and subchondral bone in vivo.

Combination of electrospinning technology with silk-montmorillonite composite hydrogel

Electrospinning technology can be combined with silk-montmorillonite composite hydrogel to prepare nanofiber scaffolds with specific structures and functions, providing a new strategy for the repair of osteochondral defects. This combination can enhance the mechanical properties and bioactivity of the scaffold and promote the integrated repair and regeneration of osteochondral defects.

Clinical transformation prospects

Silk-montmorillonite composite hydrogel has broad application value and clinical transformation prospects in the treatment of osteochondral injuries. The application of electrospinning technology can further enhance the performance of this hydrogel and improve its effectiveness in clinical treatment.

Conclusion

In summary, electrospinning technology shows great potential in the repair of osteochondral defects, especially in combination with silk-montmorillonite composite hydrogel, which provides a new solution for achieving integrated osteochondral regeneration. Future research needs to further explore the application effect of this combination in clinical treatment.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1002/adhm.202200602