Electrospinning Equipment: A novel electrospun polylactic acid silk protein patch for abdominal wall hernia repair

Application of PLA-SF composite biological hernia patch in abdominal wall hernia repair

Excellent physical properties: PLA-SF patch has high hydrophilicity and strength, and will not expand or degrade over time in vitro. Its yield point, elastic modulus and ultimate strength are 5.49 times, 51.04 times and 4.07 times that of PP mesh, and 4.82 times, 14.15 times and 4.24 times that of PLA mesh, respectively. This excellent mechanical property enables the patch to withstand the tension and pressure of the abdominal wall, provide stable support, and prevent the recurrence of hernia.

Good biocompatibility: The patch can significantly reduce abdominal inflammation and inhibit adhesion formation in a rat model. Co-culture experiments with L929 mouse fibroblasts showed that PLA-SF patch reduced the expression of inflammatory factors secreted by fibroblasts through the TGF-β1/Smad pathway and promoted fibroblast proliferation. This shows that the patch can be well compatible with body tissues and reduce the occurrence of postoperative complications.

Promote tissue remodeling and hernia repair: The degradation rate of the PLA-SF patch in vitro is slower than the degradation rate of tissue remodeling. This means that the patch can gradually degrade in vivo, while providing sufficient time and space for the growth of new tissue, promoting the repair and tissue remodeling of abdominal wall hernia.

Application of electrospinning equipment in the preparation of PLA-SF composite biological hernia patch

Preparation of nanofiber structure: Electrospinning equipment can prepare polylactic acid and silk fibroin nanofibers with specific structures and properties. These nanofibers can be used to construct the matrix structure of the patch, giving it better mechanical properties and biocompatibility. For example, nanofibers prepared by electrospinning technology can be evenly dispersed in the patch to enhance the overall performance of the material.

Regulate the microstructure of the patch: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting the concentration, viscosity, electric field strength and other parameters of the spinning solution. This allows the pore structure and pore size of the PLA-SF patch to be precisely regulated, thereby better meeting the needs of abdominal wall hernia repair. For example, a nanofiber network with appropriate porosity and uniform pore size can be prepared to provide a good microenvironment for cell adhesion, growth and tissue remodeling.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber patches loaded with bioactive molecules. For example, growth factors and drugs that promote tissue repair are loaded into polylactic acid and silk fibroin nanofibers, and the release rate is controlled to achieve the continuous effect of bioactive substances in the abdominal wall hernia repair site. This can not only enhance the biological function of the patch, but also further improve its effect in abdominal wall hernia repair.

Summary

In summary, PLA-SF composite biological hernia patch shows good application effect in abdominal wall hernia repair, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber patches with specific structures and properties can be prepared, and the performance of PLA-SF patches can be further optimized to improve its application effect in abdominal wall hernia repair.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.mtbio.2023.100915