Electrospinning Equipment: Elastic fiber-reinforced silk fibroin scaffold with double cross-linked network for human ear-shaped cartilage regeneration

Application of Fr-SF-SEAs in tissue engineering

Promote cell diffusion and uniform distribution: Fr-SF-SEAs have ideal synergistic properties of honeycomb structure, hygroscopicity and elasticity, and can significantly promote cell diffusion and achieve uniform cell distribution at high density through unconventional cyclic compression inoculation methods. This is crucial for constructing complex tissues such as human ear-shaped cartilage with uniform cell distribution and stable shape in tissue engineering.

Maintain the structural stability of regenerated cartilage: The scaffold withstands the dynamic pressure environment after subcutaneous implantation and maintains its precise original structure. Its good elasticity and fatigue durability can effectively withstand the complex mechanical environment in the body, prevent deformation and structural damage of the regenerated tissue, and ensure the normal function of the tissue.

Achieve bionic regeneration of ear-shaped cartilage: The human ear-shaped Fr-SF-SEAs scaffold maintained its original shape and size after 2 weeks of in vitro culture, and there was no obvious change in the gross appearance after 6 weeks of in vivo implantation. Histology showed that the ear-shaped structure initially formed cartilage-like tissue. This indicates that Fr-SF-SEAs achieve stable and precise ear bionic cartilage regeneration at human scale, providing the possibility for clinical applications such as microtia reconstruction.

Application of electrospinning equipment in the preparation of Fr-SF-SEAs

Preparation of nanofiber structure: Electrospinning equipment can prepare silk fibroin nanofibers with specific structure and properties. These nanofibers can be combined with silk fibers to form a composite structure with excellent mechanical properties, enhancing the elasticity and stability of Fr-SF-SEAs. For example, silk fibroin nanofibers prepared by electrospinning technology can be evenly distributed in the silk fiber network, improving the performance of the overall scaffold.

Regulating the microstructure of the scaffold: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of the Fr-SF-SEAs scaffold to be precisely regulated, thereby better meeting the needs of cell growth and tissue regeneration. For example, a nanofiber network with appropriate porosity and uniform pore size can be prepared to provide a good microenvironment for cell adhesion, growth, and migration.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. For example, growth factors and drugs that promote cartilage regeneration are loaded into silk fibroin nanofibers, and then combined with silk fibers. By controlling the release rate, the sustained action of bioactive substances in tissue engineering sites can be achieved. This can not only enhance the biological function of Fr-SF-SEAs, but also further improve its application effect in tissue engineering.

Summary

In summary, fiber-reinforced silk fiber superelastic absorbent sponge shows good application effect in tissue engineering, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber composites with specific structures and properties can be prepared, and the performance of Fr-SF-SEAs can be further optimized to improve its application effect in tissue engineering.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1007/s42765-023-00266-8