Electrospinning Equipment: Silk fibroin nanofiber hierarchical sponges accelerate healing of infected diabetic wounds via HClO self-generated cascade catalysis

Application of CSSF-P/AuGCs in the treatment of diabetic wounds

Effective absorption of exudate: CSSF-P/AuGCs have uniformly arranged microchannels in parallel, which give them excellent exudate absorption capacity, can quickly discharge exudate from the wound bed, and keep the wound clean and moist. This is crucial for the healing of diabetic wounds, because excessive exudate can easily retain bacteria and produce excessive reactive oxygen free radicals (ROS), leading to excessive inflammation and delayed wound healing.

Antibacterial and anti-inflammatory effects: AuGCs can greatly reduce the production of H2O2 from glucose in the wound, converting it into HClO through a cascade catalytic reaction to eliminate bacterial infection and reduce inflammation. In vitro experiments showed that the antibacterial activity of CSSF-P/AuGCs against Staphylococcus aureus and Escherichia coli was 92.7% and 94.27%, respectively. This highly efficient antibacterial property can effectively prevent infection of diabetic wounds and create a good microenvironment for wound healing.

Promote wound healing: Animal experiments have shown that CSSF-P/AuGC can heal diabetic wounds within 11 days, showing superior wound healing ability compared to the commercially available drug Tegaderm™. It can reduce inflammation in diabetic wounds and promote tissue repair and regeneration.

Application of electrospinning equipment in the preparation of CSSF-P/AuGCs

Preparation of nanofiber structure: Electrospinning equipment can prepare chitosan and silk protein nanofibers with specific structures and properties. These nanofibers can be used to construct more sophisticated hierarchical 3D sponge structures to further optimize their performance. For example, nanofibers prepared by electrospinning technology can be compounded with AuGCs to form composite materials with better mechanical properties and antibacterial properties.

Regulate the microstructure of the sponge: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of the CSSF-P/AuGCs sponge to be precisely regulated, thereby better meeting the needs of diabetic wound treatment.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber sponges loaded with bioactive molecules. For example, growth factors and drugs that promote wound healing are loaded into chitosan and silk fibroin nanofibers, and then combined with AuGCs to control the release rate to achieve the sustained effect of bioactive substances on diabetic wounds.

Summary

In summary, CSSF-P/AuGCs shows good application effects in the treatment of diabetic wounds, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber sponges with specific structures and properties can be prepared, and the performance of CSSF-P/AuGCs can be further optimized to improve its application effect in the treatment of diabetic wounds.

Electrospinning Nanofibers Article Source:

www.elsevier.com/ocate/carbpol