Electrospinning Equipment: Innovative application of photocoagulated silk hydrogel and scar-free regeneration research

Application of silk fibroin gel in scarless skin regeneration

Promote macrophage polarization and hair follicle stem cell recruitment: Silk fibroin gel can induce more pro-regenerative macrophages and recruit more hair follicle stem cells to the wound site. Macrophages play a key role in wound healing, and their polarization to a pro-regenerative phenotype helps to remove necrotic tissue, promote angiogenesis and cell proliferation. The recruitment of hair follicle stem cells helps the regeneration of hair follicles and the reconstruction of skin structure.

Inhibit scar formation: In a rat full-thickness skin defect model, 15% silk fibroin gel (SilMA) can significantly inhibit scar formation. This may be because silk fibroin gel reduces the transformation of fibroblasts to myofibroblasts by regulating the structure of cell populations and the expression of cytokines, thereby reducing the excessive deposition of collagen and avoiding the formation of scars.

Provide a suitable microenvironment: Silk fibroin gel has good biocompatibility and biodegradability, and can provide a suitable microenvironment for cell growth and tissue regeneration. Its porous structure is conducive to cell adhesion, migration and exchange of nutrients, and its degradation products will not have adverse reactions to the body.

Application of electrospinning equipment in the preparation of silk gel

Preparation of nanofiber structure: Electrospinning equipment can prepare silk protein nanofibers with specific structure and properties. These nanofibers can be used to construct the matrix structure of silk gel, making it have better mechanical properties and biocompatibility. For example, silk protein nanofibers prepared by electrospinning technology can be evenly dispersed in the gel to enhance the overall performance of the material.

Regulating the microstructure of the gel: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of silk gel to be precisely regulated, thereby better meeting the needs of scar-free skin regeneration. For example, a nanofiber network with appropriate porosity and uniform pore size can be prepared to provide a good microenvironment for cell adhesion, growth, and tissue remodeling.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber gels loaded with bioactive molecules. For example, growth factors and drugs that promote skin regeneration are loaded into silk fibroin nanofibers, and the release rate is controlled to achieve the continuous effect of bioactive substances at the wound site. This can not only enhance the biological function of silk fibroin gel, but also further improve its effect in scarless skin regeneration.

Summary

In summary, silk fibroin gel shows good application effect in scarless skin regeneration, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, silk fibroin nanofiber gel with specific structure and performance can be prepared, and the performance of silk fibroin gel can be further optimized to improve its application effect in scarless skin regeneration.

Electrospinning Nanofibers Article Source:

https://doi:org/10.1016/j.apmt.2021.101004