Electrospinning Equipment: Development of bioactive silk fibroin bilayer scaffolds for wound healing and scar inhibition

Application of SNF-SF-HA double-layer scaffold in the prevention and treatment of hypertrophic skin scars

Promote new tissue reconstruction and inhibit scar formation: The double-layer scaffold not only supports the reconstruction of new tissue, but also inhibits the formation of scars. It achieves scar residence by reducing wound contraction, weakening inflammatory response, and regulating collagen deposition and type conversion, which is observed in part by downregulating type I collagen, transforming growth factor-β, and α smooth muscle actin.

Maintaining moisture in the wound microenvironment: Due to the presence of SF and HA, the scaffold has high water absorption and swelling, which is conducive to the absorption of tissue fluid, maintaining moisture in the wound microenvironment, and providing good conditions for cell growth and tissue repair.

Providing a suitable cell growth microenvironment: The double-layer scaffold has excellent biocompatibility and can promote cell adhesion, growth and proliferation. Its porous structure provides sufficient space for cells, which is conducive to cell migration and the exchange of nutrients.

Application of electrospinning equipment in the preparation of SNF-SF-HA double-layer scaffolds

Preparing nanofiber structure: Electrospinning equipment can prepare silk protein nanofibers with specific structures and properties. These nanofibers can be used to construct the epidermis layer (SNF membrane) in the double-layer scaffold, giving it better mechanical properties and hydrophobicity. For example, the silk fibroin nanofibers prepared by electrospinning technology can be evenly dispersed in the epidermis to enhance the overall performance of the material.

Regulating the microstructure of the scaffold: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting the concentration, viscosity, electric field strength and other parameters of the spinning solution. This allows the pore structure and pore size of the double-layer scaffold to be precisely regulated, thereby better meeting the needs of hyperplastic skin scar prevention and treatment. For example, a nanofiber network with appropriate porosity and uniform pore size can be prepared to provide a good microenvironment for cell adhesion, growth and tissue remodeling.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. For example, growth factors and drugs that promote skin repair are loaded into silk fibroin nanofibers, and then combined with double-layer scaffolds. By controlling the release rate, the continuous effect of bioactive substances in the hyperplastic skin scar site can be achieved. This can not only enhance the biological function of the double-layer scaffold, but also further improve its effect in the prevention and treatment of hypertrophic skin scars.

Summary

In summary, the SNF-SF-HA double-layer scaffold shows good application effect in the prevention and treatment of hypertrophic skin scars, and the electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, silk protein nanofibers with specific structures and properties can be prepared, further optimizing the performance of the SNF-SF-HA double-layer scaffold and improving its application effect in the prevention and treatment of hypertrophic skin scars.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.ijbiomac.2023.128350