Electrospinning Equipment: MFGE8-loaded extracellular vesicle-derived silk fibroin nanoparticles accelerate skin ulcer healing by targeting vascular endothelial cells

Research progress of Li Changqing's team

Preparation and application of NGR-NPs@MFGE8: Li Changqing's team synthesized nanoparticles NPs through silk fibroin and loaded ferroptosis inhibitor MFGE8 to prepare NPs@MFGE8. Using silk fibroin/collagen hydrogel as a carrier, after loading NGR-NPs@MFGE8, MFGE8 in PU tissue decreased and CD13 increased. NPs@MFGE8 can inhibit mitochondrial autophagy-induced ferroptosis of vascular endothelial cells (VECs). Compared with hydrogels loaded with NPs or NPs@MFGE8, hydrogels loaded with NGR-NPs@MFGE8 continuously released CD13-targeted NGR-NPs@MFGE8 in VECs, thereby inhibiting hypoxia-induced mitochondrial autophagy and ferroptosis, and effectively accelerating wound healing in rats.

In vitro and in vivo experimental verification: In the in vitro experiment, the research team detected the expression of ferroptosis-related proteins in PU tissues by Western blotting, and found that compared with severe PU tissues, mild PU tissues showed higher levels of anti-ferroptosis-related protein GPX4 and lower levels of ferroptosis-related protein P53. In the in vivo experiment, a rat model of PU was constructed, and protein blotting and immunohistochemical staining verified that ferroptosis was positively correlated with the occurrence of PU. In addition, in vivo experiments showed that Gels-NPs had no significant difference in promoting ulcer healing compared with the gel group, but Gels-NPs@MFGE8 significantly accelerated ulcer healing after 6 days than Gels-NPs.

Application of electrospinning equipment in wound healing

Preparation of nanofiber structure: Electrospinning equipment can prepare nanofibers with specific structures and properties. These fibers can be used to construct the basic structure of wound dressings, giving them better mechanical properties and biocompatibility. For example, the large-pore nanofiber membrane has larger pores between the fibers, which is conducive to the transmission of nutrients and the excretion of waste, thereby promoting cell growth.

Regulating the microstructure of the scaffold: By adjusting the parameters in the electrospinning process, the diameter and morphology of the nanofibers can be precisely controlled, thereby optimizing the pore structure and pore size of the wound dressing and providing a good microenvironment for cell adhesion, growth and tissue remodeling.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber dressings loaded with bioactive molecules, and by controlling the release rate, the sustained action of bioactive substances at the wound site can be achieved. For example, loading growth factors and drugs that promote cell growth and differentiation into nanofibers can further improve the effect of wound healing.

Summary and Outlook

The research of Li Changqing's team provides a new method for the effective treatment of skin pressure sores. By preparing a silk fibroin/collagen hydrogel sustained-release carrier loaded with NGR-NPs@MFGE8, the ferroptosis of VECs is targeted and promoted. The application of electrospinning equipment in wound healing provides technical support for the preparation of high-performance dressings. Future research can further optimize material selection and spinning process parameters.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1186/s12951-023-02185-7