Electrospinning Equipment: Precipitation-based rapid gelation, high adhesiveness, and magnetic nanocomposite hydrogels of silk fibroin for repairing irregular bone defects

Research progress of Jiang Libo's team at Zhongshan Hospital Affiliated to Fudan University

Preparation and application of RSF/TA/Fe3O4 hydrogel: Jiang Libo's team developed a hydrogel system of regenerated silk fibroin (RSF)/tannic acid (TA)/Fe3O4 nanoparticles based on precipitation method, which has the characteristics of rapid gelation, shape adaptation, high adhesion and magnetism. This hydrogel can respond under a static magnetic field (SMF) and is used to repair irregular bone defects.

Mechanical properties and biodegradability: RSF/TA/1%Fe3O4 hydrogel exhibits good shear strength (38.4±2.94 kPa), which is similar to that of natural polymer-based RSF bioadhesive, and has excellent ductility and self-healing ability. Adding Fe3O4 nanoparticles can slow down the degradation rate of the hydrogel, showing adjustable biodegradation properties.

Biocompatibility and osteogenesis: In in vitro and in vivo experiments, RSF/TA/Fe3O4 hydrogel showed good biocompatibility and osteogenesis, especially under SMF stimulation, the osteogenesis was more significant. The potential mechanism of its osteogenesis is through the activation of cGMP/PKG/ERK signaling pathway.

Application of electrospinning equipment in the study of meniscus transplants

Preparation of nanofiber structure: Electrospinning equipment can prepare nanofibers with specific structures and properties, which can be used to construct the basic structure of meniscus scaffolds, making them have better mechanical properties and biocompatibility.

Regulating the microstructure of the scaffold: By adjusting the parameters in the electrospinning process, the diameter and morphology of the nanofibers can be precisely controlled, thereby optimizing the pore structure and pore size of the meniscus scaffold, providing a good microenvironment for cell adhesion, growth and tissue remodeling.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules, and by controlling the release rate, the continuous action of bioactive substances in the meniscus regeneration site can be achieved.

Summary and Prospect

The research of Jiang Libo's team provides a potential biomaterial for bone defect repair. Its rapid gelation and magnetic response characteristics make it have broad application prospects in clinical practice. The application of electrospinning equipment in the study of meniscus grafts provides technical support for the preparation of high-performance scaffolds. Future research can further optimize material selection and spinning process parameters, improve the biocompatibility and mechanical properties of scaffolds, and promote their clinical application.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1002/adfm.202302442