Electrospinning Equipment:MgO/MgCO₃/PCL Fibers Enhance Nerve Regeneration via Wnt Pathway 2025-03-03
This study develops a multi-gradient electrospun MgO/MgCO₃/PCL nanofiber scaffold, fabricated using an electrospinning machine, to achieve controlled Mg²⁺ release for peripheral nerve regeneration. Structural characterization confirmed the scaffold’s tunable degradation properties, while in vitro studies demonstrated that Mg²⁺ enhanced Schwann cell proliferation, migration, and repair phenotype transition. Transcriptome sequencing and western blot analysis identified the Wnt5a/β-Catenin pathway as a key regulatory mechanism. In vivo experiments using a 10 mm sciatic nerve defect model in rats showed that the 10% MgO/MgCO₃/PCL scaffold significantly improved axonal regeneration, remyelination, and functional recovery, outperforming higher Mg concentrations. This study provides a novel biomaterial strategy for long-term nerve repair, bridging electrospun fiber engineering and molecular mechanisms, and establishing a foundation for the clinical translation of magnesium-based scaffolds.
