Electrospining Machine:Three-Layer Electrospun Nanofbers Dressing with Asymmetric Wettability for Wound Healing

Background

A three-layer Nanofiber Membrane with asymmetric wettability to promote wound healing was prepared by Associate Professor Xia Dan of Hebei University of Technology, et al. Nanofiber Membrane has a hydrophilic inner layer loaded with lidocaine hydrochloride, a hydrophobic middle layer loaded with ciprofloxacin, and a hydrophobic outer layer with waterproof,Nanofiber filter media and anti-adhesion properties

The main points of this paper

1.Inspired by the structure of skin, the study developed a three-layer Nanofiber Membrane with asymmetric wettability, including a hydrophilic inner layer containing lidocaine hydrochloride (LID), a hydrophobic middle layer containing ciprofloxacin (CIP), and a hydrophobic outer layer.

2.The hydrophobic middle layer with CIP inhibits bacterial growth for a long period of time, and the hydrophilic inner layer with LID has the best water absorption capacity and air permeability.

3. TNM significantly increases the water contact angle from 10° (inner layer) to 120° (outer layer) and exhibits asymmetric wettability, which allows for the targeted delivery of wound exudate within the material while maintaining a comfortable moist environment to promote wound healing.

4. Sequential release of LID and CIP provides rapid pain relief and long-term antimicrobial effects, respectively. In addition, TNM exhibits excellent biocompatibility with L929 cells, preventing infection, accelerating epithelial regeneration, and significantly accelerating wound healing.

How does the asymmetric wettability of three-layer Nanofiber Membrane contribute to its wound healing effectiveness?

The asymmetric wettability of the three-layer Nanofiber Membrane plays a crucial role in wound healing by facilitating the targeted transport of wound exudate within the material. The hydrophobic outer layer effectively isolates external water and microbial invasion and reduces wound evaporation. This helps maintain a moist environment that is favorable for wound healing. The hydrophilic inner layer with optimal water absorption capacity promotes rapid absorption of wound exudate, while the hydrophobic middle layer maintains long-term inhibition of bacterial growth. This combination of properties of the different layers creates a synergistic effect and enhances the overall effectiveness of the dressing in promoting wound healing.

The main advantages of lidocaine hydrochloride (LID) and ciprofloxacin (CIP) in different Nanofiber Membrane are as follows:

1. Hydrophilic inner layer of lidocaine hydrochloride (LID):

(1) LID has optimal water absorption capacity to promote rapid absorption of wound exudate.

(2) LID is capable of rapidly releasing analgesic properties for effective pain relief during the initial stages of wound healing.

(3) The presence of LID supports cell proliferation and adhesion, contributing to tissue repair.

2.Hydrophobic intermediate layer ciprofloxacin (CIP).

(1) CIP maintains long-term slow-release antibacterial properties and inhibits bacterial growth over a longer period of time.

(2) The antimicrobial properties of CIP help prevent infection and promote a sterile environment for wound healing.

By incorporating CIP in the middle layer, Nanofiber Membrane ensures continuous antimicrobial effect while maintaining a waterproof and protective barrier.

Originallink: https://doi.org/10.1016/j.nanoms.2024.01.008