Electrospinning Device: Co-assembly of designer peptides and silk cellulose into hybrid nanofiber gels for neural regeneration after spinal cord injury

Background

Electrospinning technology can prepare nanofibers with high specific surface area and high porosity, which are very important for cell ingrowth and tissue regeneration. The F-SAP/SF hybrid hydrogel reported by the research team of Sun Yat-sen University, through electrostatic interaction and osmotic pressure difference, SF micelles rearranged on the F-SAP nanofiber network and formed rod-like filaments, which helped to provide a structure similar to the natural extracellular matrix (ECM) and promote nerve regeneration.

Enhancement of mechanical properties

Spectral analysis showed that the conformational change of SF from random coil to β sheet helped to enhance the mechanical properties of the resulting hybrid hydrogel. Electrospinning technology can further enhance the mechanical properties of hydrogels by regulating the arrangement and cross-linking of fibers, providing a stable scaffold for nerve regeneration.

Promotion of nerve regeneration

F-SAP/SF hybrid hydrogel combined with controlled release of NT-3 provides a relaxed environment for nerve regeneration by providing a nanofiber substrate for axon regeneration, inflammation regulation and myelin regeneration, thereby improving motor and electrophysiological performance. Electrospinning technology can be used to prepare drug-loaded fibers, achieve controlled release of drugs, and promote nerve regeneration.

Biocompatibility and biodegradability

Biocompatibility refers to the compatibility between a material and a host, while biodegradability refers to the ability of a material to degrade and be absorbed or excreted in the body. F-SAP/SF hybrid hydrogels have good biocompatibility, low immunogenicity, and biodegradability, which are essential for long-term scaffold materials implanted in the body.

Advantages of dual network structure

The dual network structure formed by F-SAP/SF hydrogels can mimic the ECM architecture, and combined with the independent and adjustable biochemical properties of F-SAP and SF, it provides a platform for the discovery of high-performance materials and is also conducive to clinical transformation. Electrospinning technology can prepare hydrogels with a similar dual network structure by regulating the cross-linking and network structure of fibers, thereby enhancing their mechanical properties and biological functions.

Potential for clinical transformation

As a long-term in vivo scaffold, F-SAP/SF hybrid hydrogels provide a new perspective for SCI treatment. Nanofiber scaffolds prepared by electrospinning technology are expected to be used in clinical treatment due to their excellent biocompatibility and biodegradability

Summary

In summary, there is a close connection between electrospinning technology and the application of silk fibroin in the treatment of spinal cord injury. Electrospinning technology can prepare silk fibroin-based nanofibers with specific properties. These nanofibers show excellent biocompatibility, mechanical properties and nerve regeneration promotion ability in the treatment of spinal cord injury. These studies provide new ideas and methods for the clinical treatment of spinal cord injury.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1126/sciadv.adg0234