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Professor Zhao Bin's team synthesized RSFMA by modifying silk fibroin with glycidyl methacrylate, and synthesized MeHA by grafting methacrylic anhydride on the hyaluronic acid skeleton. The two components were combined and an appropriate amount of photoinitiator was added to form a hydrogel scaffold under ultraviolet light. This UV-driven cross-linking system provides a hydrogel system with controllable gelation time and space, which can well fill complex root canal systems.
Analysis of infrared spectra and nuclear magnetic resonance hydrogen spectra confirmed that silk fibroin and hyaluronic acid were successfully methacrylated, and the grafting rate of MeHA reached 40.7%. The gelation time was greatly affected by the concentration of MeHA. The concentration of MeHA was selected as 1%, and the gelation time was controlled to about 5 minutes.
Studies have shown that RSFMA/MeHA composite hydrogel has good biocompatibility and low cytotoxicity, and can well promote the proliferation and differentiation of dental pulp stem cells.
The results of rheological analysis showed that the stiffness of MeHA hydrogels could be enhanced by adding RSFMA. The degradation rate of hydrogels also changed with the copolymerization ratio. As the concentration of RSFMA increased, the degradation rate of copolymerized hydrogels slowed down.
The research results of Professor Zhao Bin's team demonstrated that the photocrosslinked RSFMA/MeHA composite hydrogels have injectability, adjustable mechanical properties and high porosity, which have great application potential in pulp regeneration.
Electrospinning technology can prepare nanofiber scaffolds with high porosity and high specific surface area, which are similar to the high porosity and excellent mechanical properties of RSFMA/MeHA composite hydrogels. The application of electrospinning technology in pulp regeneration has also been widely studied. It can simulate the structure of natural extracellular matrix and promote tissue regeneration. RSFMA/MeHA composite hydrogel and electrospinning technology have potential synergistic application prospects in the preparation of high-performance biomedical materials. Both can be used to construct biomaterial scaffolds suitable for dental pulp regeneration, especially in simulating the structure and function of natural dental pulp tissue.
In summary, the RSFMA/MeHA composite hydrogel developed by Professor Zhao Bin's team shows great potential in the field of dental pulp tissue engineering, and has similar advantages and application prospects as electrospinning technology in the preparation of high-performance biomedical materials.
Electrospinning Nanofibers Article Source:
https://doi.org/10.1016/j.ijbiomac.2022.06.087
