Electrospinning Device: Silk fibroin-based hemostatic powder has instant and strong adhesion properties for seamless closure of gastrointestinal defects

Background

SF/PEG/TA powders were obtained by mixing silk fibroin (SF) solution and polyethylene glycol (PEG) solution, adding tyrosine (TA) aqueous solution to induce the structural transformation of SF, and then grinding at low temperature. This powder can rapidly expand into microgels after absorbing interfacial liquids, and transform into macroscopic uniform hydrogels in situ, enhancing its interfacial bonding with various substrates in a fluid environment.

Wet Adhesion Performance of SF/PEG/TA Powders

SF/PEG/TA powders can be instantly assembled into bulk hydrogels due to multiple reversible dynamic interactions between different components. The presence of PEG components significantly improves the surface wettability and water absorption of the powders, making SF/PEG/TA powders have excellent self-melting ability and cohesion under swelling and wet conditions.

In vitro Tissue Adhesion Performance of SF/PEG/TA Powders

SF/PEG/TA powders can directly form fast and tough adhesion with wet tissues within 3.5 seconds, showing strong tissue adhesion and the ability to close incisions instantly.

In vivo hemostatic sealing performance of SF/PEG/TA powder

In multiple in vivo rat bleeding models, SF/PEG/TA powder showed superior hemostatic sealing ability and could effectively control irregular shaped and incompressible bleeding tissue.

In vivo skin incision sealing ability of SF/PEG/TA powder

SF/PEG/TA powder can effectively promote skin wound healing in vivo, showing strong wet tissue adhesion and rapid hemostatic sealing ability.

In vivo gastrointestinal injury sealing ability of SF/PEG/TA powder

SF/PEG/TA powder can effectively achieve rapid sealing and healing of severe gastrointestinal injuries in vivo, showing the ability to promote wound healing and tissue regeneration.

Connection with electrospinning technology

Electrospinning technology can be used to prepare nanofibers with specific functions, which can be used to enhance the performance of hemostatic materials, such as by doping hemostatic agents in nanofibers or using the physical properties of nanofibers to inhibit blood flow. The study of SF/PEG/TA powder provides a new type of hemostatic material, and electrospinning technology can be further used to develop new forms of this material, such as hemostatic nanofiber membranes or bandages, to enhance its hemostatic effect and biocompatibility.

In summary, SF/PEG/TA powder, as a new type of silk fibroin-based hemostatic powder, has rapid hemostasis and strong adhesion properties, and has potential synergistic application prospects with electrospinning technology in the preparation of high-performance biomedical materials.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1039/d4tb00554f