Electrospinning Device: Preparation of silk fibroin/alginate composite nanofiber scaffolds for biomedical applications by thermally induced phase separation (TIPS)

Application of SF/SA composite nanofiber scaffolds in tissue engineering

Simulation of natural extracellular matrix structure: The scaffold successfully prepared a nanofiber structure through thermal phase separation, with a porosity of more than 90%, which can well simulate the natural fiber structure of the tissue extracellular matrix. This structure is conducive to cell adhesion, growth and migration, and provides a good microenvironment for tissue engineering.

Promote cell growth and proliferation: Cell culture results show that MG-63 osteoblasts can adhere and grow well on the surface of SF/SA scaffolds. This shows that the scaffold has good cell compatibility, can provide suitable conditions for cell growth and proliferation, and promote tissue repair and regeneration.

Good physical properties: There are physical interactions between SF and SA molecules, such as electrostatic interactions and hydrogen bonds between amino and COO− groups. This interaction makes the scaffold have good mechanical properties and stability, can withstand certain mechanical loads, and provide stable support for tissue engineering.

Application of electrospinning equipment in the preparation of SF/SA composite nanofiber scaffolds

Preparation of nanofiber structure: Electrospinning equipment can prepare silk fibroin and sodium alginate nanofibers with specific structures and properties. These nanofibers can be used to construct more sophisticated composite scaffold structures and further optimize their performance. For example, nanofibers prepared by electrospinning technology can be composited with SF/SA to form composite materials with better mechanical properties and biocompatibility.

Regulating the microstructure of the scaffold: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of the SF/SA composite nanofiber scaffold to be precisely regulated, thereby better meeting the needs of tissue engineering.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. For example, growth factors and drugs that promote tissue repair are loaded into silk fibroin and sodium alginate nanofibers, and then combined with composite scaffolds. By controlling the release rate, the continuous effect of bioactive substances in tissue engineering sites can be achieved.

Summary

In summary, the SF/SA composite nanofiber scaffold shows good application effects in tissue engineering, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber scaffolds with specific structures and properties can be prepared, and the performance of SF/SA composite nanofiber scaffolds can be further optimized to improve their application effect in tissue engineering.

Electrospinning Nanofibers Article Source:

http://dx.doi.org/10.1016/j.msec.2015.05.052