Electrospinning Device: Protective double-sided nanofiber dressing made from mussel and silk protein with multifunctionality of local hemostasis and antimicrobial penetration

Background

This study aims to develop a local hemostatic dressing with dual functions of hemostasis and anti-adhesion, which is prepared by combining bioengineered mussel adhesive protein (MAP) and silk fibroin (SF) for electrospinning. The inner side of this dressing contains MAP containing DOPA, which can promote blood coagulation and tissue adhesion; the outer side is composed of hydrophobic SF, which has anti-adhesion and physical protection functions.

Research content and methods

In the study, MAP containing DOPA (dcMAP) was used to form an inner nanofiber layer in contact with the bleeding site to achieve hemostasis and wound tissue adhesion. SF was used to make the outer nanofiber layer, and the β-sheet content of its crystalline domain was increased by methanol treatment and heat treatment, making the surface sufficiently hydrophobic to prevent contaminant penetration.

In vitro and in vivo evaluation

Through in vitro experiments, the researchers evaluated the hemostatic ability of MAP biomaterials and found that the dcMAP group was able to aggregate and activate platelets, thereby promoting blood coagulation. In a rat liver injury model, this double-sided nanofiber dressing showed significantly reduced clotting time and blood loss, confirming its effectiveness as a local hemostatic agent.

Biocompatibility and biodegradability

Since both MAP and SF proteins are completely biodegradable and biocompatible in vivo, the dressing is suitable for staying in the body. The study also confirmed that the dressing can be biodegraded into non-toxic degradation products, meeting the requirements of absorbable biomaterials.

Summary and Outlook

This study successfully developed a double-sided nanofiber membrane based on two protein biomaterials as a local hemostatic dressing with hemostatic and anti-adhesion multifunctionality. This dressing has great application prospects in internal bleeding care applications, especially in biologically favorable materials that need to be applied after surgery and remain in the body.

In summary, this study provides a new type of electrospun nanofiber hemostatic dressing, which combines the advantages of mussel adhesion protein and silk fibroin, which can not only effectively stop bleeding, but also prevent pollutant penetration, and has good biocompatibility and biodegradability, providing a new direction for the development of clinical hemostatic materials.