Electrospinning Device: Silk fibroin-based nanofiber scaffolds combined with nanoengineered live bacteria can accelerate healing of infected wounds without antibiotics

Background

Electrospinning is an efficient, low-cost and well-controlled method for preparing nanofiber materials. This technology allows charged polymer solutions to flow and deform in an electrostatic field, and solidify through solvent evaporation or melt cooling to obtain fibrous materials. In this study, nanofiber scaffolds based on silk fibroin were prepared by electrospinning technology. This scaffold has a high specific surface area and a porous structure, which is conducive to cell attachment, proliferation and differentiation.

Biocompatibility and bioactivity

Nanofibers prepared by electrospinning technology have broad application prospects in the biomedical field due to their unique physical properties and surface morphology. As a natural material, silk fibroin has good biocompatibility and bioactivity. The silk fibroin nanofiber scaffold prepared by electrospinning technology maintains these properties and shows excellent biocompatibility in this study.

Antibacterial properties

Electrospinning technology can prepare nanofibers with antibacterial properties. In this study, the silk fibroin nanofiber scaffold equipped with nanoengineered TA-Fe(III) chelate-encapsulated Lactobacillus reuteri exhibited excellent antibacterial properties. This antibacterial property is essential for accelerating the healing of bacterially infected wounds.

Environmental resistance

The nanofiber scaffolds prepared by electrospinning technology can enhance their environmental resistance through special nanoengineering methods, such as TA-Fe(III) chelate encapsulation. In this study, Mbr/L@FeTA can maintain satisfactory bioactivity even when used with antibiotics, which shows the stability and effectiveness of electrospun scaffolds in complex environments.

Promoting wound healing

The nanofiber scaffolds prepared by electrospinning technology can mimic the structure of the extracellular matrix, promote cell growth and proliferation, inhibit bacterial growth at the wound site, and accelerate the wound healing process. In this study, the Mbr/L@FeTA scaffold significantly improved the wound microenvironment and regulated the inflammatory response by stably releasing organic acids and other metabolites, thereby promoting wound healing.

Clinical application potential

The nanofiber scaffolds prepared by electrospinning technology show great potential in clinical applications. The silk fibroin-based nanofiber scaffold Mbr/L@FeTA proposed in this study provides a novel and effective method for antibiotic-free infected wound treatment and has the potential for wide clinical application.

Summary

In summary, electrospinning technology plays a key role in the preparation of nanofiber scaffolds with specific biocompatibility, antibacterial properties and the ability to promote wound healing, which provides a solid technical foundation for the application of silk fibroin-based nanofiber scaffolds in accelerating the healing of bacterial-infected wounds without antibiotics.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.cej.2024.154233