Electrospinning Device: Silk fibroin-DNA hydrogel-based cartilage organoid precursors promote cartilage repair

Background

Electrospinning technology is widely used in cartilage tissue engineering to prepare nanofiber scaffolds with extracellular matrix (ECM)-like structures, which can promote cell growth and tissue regeneration. Similarly, RSD-MS, as a new type of hydrogel microsphere, provides a favorable microenvironment for bone marrow mesenchymal stem cells (BMSCs) by mimicking the structure and function of ECM, promoting their proliferation, adhesion and chondrogenic differentiation, thus playing a role in the treatment of osteoarthritis.

Physical and chemical properties of nanofiber scaffolds

Electrospinning technology can prepare nanofibers with adjustable physical and chemical properties, which are essential for cartilage repair and regeneration. RSD-MS hydrogel microspheres also exhibit adjustable physical and chemical properties, showing promise in cartilage tissue engineering by replicating the natural microenvironment.

Promote cell growth and differentiation

Electrospinning nanofiber scaffolds can promote cell adhesion and growth due to their high specific surface area and porous structure. RSD-MS hydrogel microspheres can also enhance the proliferation, adhesion and chondrogenic differentiation of BMSCs, which is essential for cartilage repair and osteoarthritis treatment.

Transcriptome analysis and gene expression regulation

Electrospinning nanofiber scaffolds can affect cell behavior by regulating gene expression. Transcriptome analysis of RSD-MSs showed that they induced chondrogenesis mainly through integrin-mediated adhesion pathways and biosynthesis of glycosaminoglycans, which is similar to the mechanism of electrospinning nanofiber scaffolds in regulating cell behavior.

In vivo cartilage repair ability

Nanofiber scaffolds prepared by electrospinning technology have shown potential in in vivo cartilage repair. Studies on RSD-MSs in rat cartilage defect models showed that they can significantly promote cartilage regeneration, which is consistent with the effect of electrospinning nanofiber scaffolds in in vivo cartilage repair.

Innovative strategies and material selection

Electrospinning technology provides an innovative strategy and material selection for cartilage regeneration and tissue engineering. As a new material, RSD-MSs also provides an innovative strategy for the construction of cartilage organoids, which indicates that electrospinning technology and RSD-MSs have similar application prospects and potential in the treatment of osteoarthritis.

Summary

In summary, electrospinning technology and silk fibroin-DNA-based hydrogel microspheres (RSD-MS) have shown the ability to promote cartilage repair and regeneration in the treatment of osteoarthritis. They provide a favorable microenvironment for BMSCs by simulating the structure and function of ECM, thus playing an important role in the treatment of osteoarthritis. These two technologies have broad application prospects in cartilage tissue engineering and regenerative medicine, providing new directions for future research and clinical applications.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.bioactmat.2024.02.016