Electrospinning Device: Silk fibroin has the function of regulating endothelial cell proliferation

Preparation of electrospun silk fibroin nanofibers

Studies have shown that silk fibroin nanofibers can be prepared by electrospinning technology. These fibers are stretched to a high degree under the action of an electric field and dried naturally in the air, imitating the process of silkworms spinning silk. This technology can prepare silk fibroin-based nanofibers with specific structures and functions, providing a material basis for studying their effects on endothelial cells.

Effect of silk fibroin on endothelial cell proliferation

Silk fibroin nanofibers prepared by electrospinning can promote the growth and proliferation of endothelial cells. This was confirmed by the results of CCK-8 assay and flow cytometry. This echoes the results of the research team led by Qiu Juhui that silk fibroin promotes cell growth and proliferation by inducing chromatin reorganization and H3K9me3 modification.

Effect of silk fibroin on H3K9me3 levels

The research of Qiu Juhui's team found that the H3K9me3 level in endothelial cells treated with silk fibroin was significantly increased, while the histone deacetylase inhibitor TSA could inhibit the H3K9me3 level, thereby reducing cell proliferation. This suggests that electrospun silk fibroin nanofibers may affect the epigenetic state of endothelial cells through similar mechanisms, thereby regulating cell behavior.

Effect of silk fibroin on chromatin reorganization

The research of Qiu Juhui's team also found that silk fibroin treatment can promote the accumulation of H2B-marked chromatin in the nuclear lumen of endothelial cells, indicating that the upregulation of H3K9me3 in EC after silk fibroin treatment may be related to chromatin reorganization related to cell proliferation. This is consistent with the effect of electrospun silk fibroin nanofibers on the chromatin structure of endothelial cells.

Biomedical applications of electrospun silk fibroin nanofibers

Due to their unique surface morphology and biocompatibility, electrospun silk fibroin nanofibers have a wide range of applications in the biomedical field, including as tissue engineering scaffolds and drug delivery systems. These applications may involve the regulation of the epigenetic state of endothelial cells by silk fibroin, thereby affecting cell function and tissue regeneration.

Summary

In summary, the combination of electrospinning technology and silk fibroin provides a new way to study the epigenetic effects of silk fibroin on endothelial cells, especially in regulating endothelial cell proliferation, H3K9me3 levels and chromatin reorganization. This combination not only helps to deeply understand the biological effects of silk fibroin, but also provides the possibility for the development of new therapeutic strategies.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1063/5.02038.58