Electrospinning Device: Silk protein biomimetic glue: a revolutionary tissue adhesive that helps efficient transdermal drug delivery

Background

In transdermal drug delivery system (TDDS) patches, achieving long-term adhesion, high drug loading, and rapid drug release simultaneously is a major challenge. The connection between electrospinning technology and mulberry silk and non-mulberry silk in this field is mainly reflected in the following aspects

Preparation of high-performance adhesives

The new adhesive PHT-SP-Cu2+ can be synthesized by electrospinning technology in combination with polyethylene glycol (PEG), hexamethylene diisocyanate (HDI), trimethylolpropane (TMP) and silk protein (SP). This adhesive is combined with Cu2+ to improve the adhesion, drug loading and drug release of the patch.

Structural and performance characterization

The structure of the adhesion chain in PHT-SP-Cu2+ and the formation of the Cu-p-π conjugated network were confirmed using characterization techniques such as Fourier transform infrared spectroscopy (FT-IR), 13C NMR, XPS, SEM imaging and thermodynamic evaluation, which are crucial for improving the performance of the adhesive.

Enhanced adhesion performance

PHT-SP-Cu2+ adhesive prepared by electrospinning technology showed 7.6 times higher peel strength than the commercial adhesive DURO-TAK® 87–4098 in pig skin peeling test, and can adhere to the human body for more than six days, showing excellent adhesion performance.

Improve drug compatibility and release efficiency

PHT-SP-Cu2+ adhesive showed improved drug compatibility, promoted drug release and enhanced skin penetration using ketoprofen as a model drug. Its drug loading increased by 6.25 times compared with PHT, and also increased by 19.22 times in pharmacokinetic analysis.

Regulating drug release mechanism

The mechanism of α-helix promoting drug release was proved by Flori-Hawkins interaction parameters, molecular dynamics simulation and FT-IR. This is the first controlled release method applied in TDDS by electrospinning technology combined with mulberry silk and non-mulberry silk.

Biosafety Assessment

The biosafety assessment highlighted the excellent skin cell compatibility and safety of PHT-SP-Cu2+ in transdermal applications, which is essential to ensure the safety of TDDS patches in clinical applications.

Summary

In summary, the combination of electrospinning technology with mulberry silk and non-mulberry silk provides a new strategy for TDDS patches, which achieves high adhesion, high drug loading, and rapid drug release by regulating the structure and properties of the adhesive, while ensuring good biocompatibility and safety. This study not only solves the existing challenges in TDDS, but also opens up new avenues for the development of adhesives with excellent adhesion, drug loading, and release efficiency.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.actbio.2024.04.024