Electrospinning Equipment: Double-layer silk fibroin/sodium alginate scaffold promotes full-thickness wound vascularization

Application of PM@SASFm double-layer scaffold in the treatment of full-thickness skin defects

Simulating the layered structure of human skin: The PM@SASFm double-layer scaffold consists of a non-porous SASFm membrane and a porous PM scaffold. Its structure mimics the layering of human skin, in which the SASFm membrane is similar to the epidermis and provides a barrier function, while the PM scaffold is similar to the dermis and has good mechanical properties and sensory detection functions.

Promoting vascularization and inflammatory phase advancement: Studies have shown that the PM@SASFm scaffold can promote angiogenesis, increase the number of new blood vessels, and provide sufficient blood supply and nutrients for wound healing. At the same time, it can also advance the inflammatory phase, promote the removal of inflammatory cells and the recruitment of repair cells, and accelerate the wound healing process.

Good biodegradability and biocompatibility: The double-layer scaffold exhibits appropriate biodegradability and can gradually degrade according to the progress of wound healing, avoiding the problem of frequent replacement of traditional dressings and reducing the economic and psychological burden on patients. In addition, it also has good biocompatibility and does not have a negative impact on cell proliferation and vitality.

Application of electrospinning equipment in the preparation of PM@SASFm double-layer scaffolds

Preparation of nanofiber structure: Electrospinning equipment can prepare silk protein nanofibers with specific structures and properties. These nanofibers can be used to construct SASFm membranes in double-layer scaffolds, giving them better mechanical properties and barrier functions. For example, silk protein nanofibers prepared by electrospinning technology can be composited with sodium alginate to form a composite membrane with better performance.

Regulating the microstructure of the scaffold: Electrospinning technology can accurately control the diameter and morphology of nanofibers by adjusting parameters such as the concentration, viscosity, and electric field strength of the spinning solution. This allows the pore structure and pore size of the double-layer scaffold to be precisely regulated, thereby better meeting the needs of full-thickness skin defect treatment. For example, a nanofiber network with appropriate porosity and uniform pore size can be prepared to provide a good microenvironment for cell adhesion, growth, and the formation of new tissues.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. For example, growth factors and drugs that promote skin repair are loaded into silk protein nanofibers, and then combined with double-layer scaffolds. By controlling the release rate, the sustained effect of bioactive substances in the full-thickness skin defect site can be achieved. This can not only enhance the biological function of the double-layer scaffold, but also further improve its effect in the treatment of full-thickness skin defects.

Summary

In summary, the double-layer scaffold (PM@SASFm) shows good application effects in the treatment of full-thickness skin defects, and the electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber scaffolds with specific structures and properties can be prepared, and the performance of PM@SASFm double-layer scaffolds can be further optimized to improve its application effect in the treatment of full-thickness skin defects.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1088/1758-5090/ac73b7