Electrospinning Equipment: Novel magnetic silk-fibrin scaffold with delayed degradation has potential for long-distance vascular repair

Application of MSFCs in long-distance vascular repair

Delayed degradation properties: MSFCs have significant delayed degradation properties, which is mainly attributed to the newly formed hydrogen bonds between iron-based magnetic nanoparticles (MNPs) and silk fibroin scaffolds (SFCs), and the dual role of iron atoms inside MNPs complexing with tyrosine (the active center of proteolytic enzymes) to inhibit the activity of proteolytic enzymes. This delayed degradation property enables MSFCs to maintain structural stability for a long time in vivo, providing continuous support for long-distance vascular repair.

Enhanced mechanical properties: Due to the introduction of MNPs, the crystallinity, magnetocaloric properties and mechanical strength of MSFCs have been significantly improved. These enhanced mechanical properties enable MSFCs to withstand blood flow pressure and mechanical stress in blood vessels, ensuring that no structural damage or deformation occurs during long-distance vascular repair.

Promote endothelial cell proliferation: MSFCs can significantly promote the growth of CD34-labeled vascular endothelial cells (VECs). This is crucial for long-distance vascular repair, because the proliferation and migration of VECs can accelerate the regeneration of vascular endothelium and restore the integrity and function of blood vessels.

Application of electrospinning equipment in the preparation of MSFCs

Preparation of nanofiber structure: Electrospinning equipment can prepare silk protein nanofibers with specific structures and properties. These nanofibers can be composited with MNPs to form magnetic silk protein nanofibers with excellent mechanical properties and magnetic response properties. Through electrospinning technology, the diameter and morphology of nanofibers can be precisely controlled, thereby optimizing the microstructure and performance of MSFCs.

Regulating the microstructure of the scaffold: By adjusting the parameters such as spinning solution concentration, viscosity, and electric field strength during the electrospinning process, the pore structure and pore size of magnetic silk protein nanofibers can be precisely regulated. This enables the pore structure of MSFCs to better meet the growth and migration needs of vascular endothelial cells and provide a suitable microenvironment for long-distance vascular repair.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. For example, growth factors and drugs that promote vascular repair are loaded into magnetic silk protein nanofibers, and the release rate is controlled to achieve the continuous effect of bioactive substances at the vascular repair site. This can not only enhance the biological function of MSFCs, but also further improve its effect in long-distance vascular repair.

Summary

In summary, the magnetic silk protein scaffold shows good application effect in long-distance vascular repair, and the electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, magnetic silk protein nanofibers with specific structure and properties can be prepared, further optimizing the performance of MSFCs and improving its application effect in long-distance vascular repair.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1016/j.bioactmat.2021.04.036