Electrospinning Equipment: Effects of type I collagen/silk fibroin scaffolds with different pore sizes on the proliferation and differentiation of human dental pulp cells

Application of CSF scaffolds in the regeneration of dentin-pulp complex

Promote the proliferation and differentiation of human dental pulp cells (HDPC): CSF scaffolds can simulate 3D microenvironment and provide suitable growth and differentiation conditions for HDPC. Studies have shown that scaffolds with pore sizes of 421±27μm and 579±36μm can enhance the adhesion and ALP activity of HDPCs more than scaffolds with pore sizes of 707±43μm. This indicates that CSF scaffolds can effectively promote the proliferation and odontogenic differentiation of HDPCs, providing a cellular basis for the regeneration of dentin-pulp complexes.

Good biocompatibility and mechanical properties: CSF scaffolds have good biocompatibility and are well compatible with HDPCs without causing obvious cytotoxicity. At the same time, its mechanical properties can also meet the needs of dentin-pulp complex regeneration and provide stable support for the growth and remodeling of new tissues.

Application of electrospinning equipment in the preparation of CSF scaffolds

Preparation of nanofiber structure: Electrospinning equipment can prepare type I collagen and silk protein nanofibers with specific structures and properties. These nanofibers can be used to construct the basic structure of CSF scaffolds, giving them better mechanical properties and biocompatibility. For example, nanofibers prepared by electrospinning technology can be evenly dispersed in the scaffold to enhance the overall performance of the material.

Regulating the microstructure of the scaffold: By adjusting the parameters such as the spinning solution concentration, viscosity, and electric field strength during the electrospinning process, the diameter and morphology of the nanofibers can be precisely controlled. This allows the pore structure and pore size of the CSF scaffold to be precisely regulated, thereby better meeting the needs of dentin-pulp complex regeneration. For example, a nanofiber network with appropriate porosity and uniform pore size can be prepared to provide a good microenvironment for cell adhesion, growth, and tissue remodeling.

Loading bioactive substances: Electrospinning equipment can prepare nanofiber scaffolds loaded with bioactive molecules. For example, growth factors and drugs that promote the growth and differentiation of dental pulp cells are loaded into type I collagen and silk fibroin nanofibers, and the release rate is controlled to achieve the continuous effect of bioactive substances in the regeneration site of the dentin-pulp complex. This can not only enhance the biological function of CSF scaffolds, but also further improve their effect in the regeneration of dentin-pulp complexes.

Summary

In summary, type I collagen/silk fibroin scaffolds show good application effects in the regeneration of dentin-pulp complexes, and electrospinning equipment provides strong technical support for its preparation. Through electrospinning technology, nanofiber scaffolds with specific structures and properties can be prepared, further optimizing the performance of CSF scaffolds and improving their application effects in the regeneration of dentin-pulp complexes.

Electrospinning Nanofibers Article Source:

https://doi.org/10.1093/rb/rbab028